农业资源与环境学报2024，Vol.41Issue(3)：697-705,9.DOI:10.13254/j.jare.2023.0298
东洞庭湖表层沉积物中抗生素及抗性基因的时空分异特征
Spatio-temporal differentiation of antibiotic and antibiotic resistance genes in surface sediments of East Dongting Lake basin
摘要
Abstract
In order to study the spatio-temporal differentiation of antibiotics and antibiotic resistance genes(ARGs)in the East Dongting Lake,we collected sediment samples from the East Dongting Lake basin in wet and dry seasons and studied 4 classes of 12 antibiotics and 8 corresponding resistance genes using ultra-high performance liquid chromatography-tandem mass spectrometry and real-time fluorescence quantitative PCR.The results showed that the antibiotic concentration level in sediments was ng·g-1,and the detection rate and concentration level of 12 antibiotics in 4 classes were higher in the dry season than in the wet season.The sediment concentration ranges from ND to 176.94 ng·g-1 in the wet season,with an average concentration of 9.75 ng·g-1.In the dry season,the concentration range was ND-101.34 ng·g-1,and the average concentration was 10.88 ng·g-1.Sulfonamides and quinolones are the main antibiotics in East Dongting Lake.A total of 8 ARGs were detected in sediment samples during the wet period,and the absolute abundance range was ND-1.03×107 copies·g-1.The absolute abundance in the dry season ranged from 9.93×102-6.32×109 copies·g-1.Spatially,the total abundance of ARGs downstream was higher than that in the upstream.Antibiotics and ARGs in sediments showed a certain correlation,and the positive correlation was better in the dry season.Our results indicated that the concentration of antibiotics and ARGs in the surface sediments of East Dongting Lake varies significantly with time and space.The pollution level is higher in the dry season and the area close to aquaculture.关键词
东洞庭湖流域/抗生素/抗生素抗性基因/时空分异Key words
East Dongting Lake basin/antibiotic/antibiotic resistance genes/spatio-temporal differentiation分类
资源环境引用本文复制引用
陈金明,卢少勇,葛飞,王永强,李琳琳,杨莉园,秦海娇,蒋昀耕..东洞庭湖表层沉积物中抗生素及抗性基因的时空分异特征[J].农业资源与环境学报,2024,41(3):697-705,9.基金项目
国家自然科学基金项目(41877409) （41877409）
国家环境保护湖泊污染控制重点实验室开放基金(2022HPZD-04) （2022HPZD-04）
岳阳市长江水生态环境保护研究项目(2022-LHYJ-02-0207) National Natural Science Foundation of China(41877409) （2022-LHYJ-02-0207）
Open Research Fund of State Environmental Protection Key Laboratory for Lake Pollution Control(2022HPZD-04) （2022HPZD-04）
Study on Water Ecological Environment Protection of Yangtze River in Yueyang City(2022-LHYJ-02-0207) （2022-LHYJ-02-0207）
