食品与发酵工业2024，Vol.50Issue(9)：203-209,7.DOI:10.13995/j.cnki.11-1802/ts.035254
干腌火腿活性肽的抗氧化性能研究与鉴定
Study and identification of antioxidant properties of dry-cured ham active peptides
摘要
Abstract
Taking Jinhua ham as the research object,the crude peptide of ham was preliminary separation was carried out by means of gel chromatography to determine the antioxidant capacity of each component.The samples were analyzed by liquid chromatography tan-dem mass spectrometry equipped with an online sodium jet ion source.The whole system was the Orbitrap Q-Exactive Plus mass spectrome-ter with EASY-nanoLC 1200 in series to identify the sequence of active peptides in Jinhua ham,and 31 short peptides with high abun-dance and unidentified were found.PeptideRanker was used for prediction analysis,and three peptides with high abundance and activity were obtained.The amino acid sequence was DHDGPDHW,FPPDVGD,and PFGDTH.Using DPPH free radical scavenging capacity,ABTS cationic scavenging capacity,and·OH free radical scavenging capacity to test antioxidant capacity,it was proved that the Jinhua ham crude peptide solution had good antioxidant capacity.The infrared spectrum showed that the crude peptide had absorption bands from 1 600 cm-1 to 1 700 cm-1.Through the synthesis of short peptides,the antioxidant capacity of the peptide was verified.Molecular docking experiments showed that the superoxide dismutase enzyme had binding capacity with DHDGPDHW,FPPDVGD,and PFGDTH.The FPP-DVGD had the strongest binding capacity of-6.6 kcal/mol.The experiment showed that FPPDVGD had a good antioxidant capacity and could be used as a natural antioxidant in food,medicine,or cosmetics.It has a broad application prospect and provides theoretical support for further developing and utilizing dry-cured products.关键词
干腌火腿/抗氧化肽/凝胶层析色谱/液相色谱-质谱联用/分子对接Key words
dry-cured ham/antioxidant peptides/gel chromatography/nano-HPLC-MS/MS/molecular docking引用本文复制引用
杨天志,张迎阳,邹平,董亚云,李锦,高蕙文,耿成钢..干腌火腿活性肽的抗氧化性能研究与鉴定[J].食品与发酵工业,2024,50(9):203-209,7.基金项目
江苏省科技项目(BY2021215) （BY2021215）
常州市科技项目(CE20222029) （CE20222029）
