现代妇产科进展2024，Vol.33Issue(5)：362-368,7.DOI:10.13283/j.cnki.xdfckjz.2024.05.006
一种对激素具有反应性的大鼠阴道类器官模型建立及鉴定
Establishment and identification of a hormone-responsive rat vaginal organoid model
张莉 1范琳媛 1张心 1李婷 1张展 1宗晓楠 1白会会 1刘朝晖1
作者信息
- 1. 首都医科大学附属北京妇产医院/北京妇幼保健院,北京 100026
- 折叠
摘要
Abstract
Objective:To construct a physiologically relevant rat vaginal organoid mod-el responsive to hormone.Methods:Rat vaginal tissues were isolated and disintegrated into cell suspension and coated with Matrigel,then cultured with organoid medium.HE staining and im-munofluorescence were performed to compared organoid with the original tissue in the morpho-logical characteristics and characterize keratin and proliferation marker in vaginal epithelial cells.Estrogen and progesterone treatment were performed to verify the responsiveness of or-ganoid to hormones.Results:The rat vaginal organoids were solid spheres with a diameter of a-bout 100μm.HE staining showed that it was composed of multiple layers of epithelial cells,and immunofluorescence staining showed that the expressions of vaginal epithelial markers such as Krt5/8,Krt15,Ki67 and TP63 were basically consistent with the original tissue.The mRNA lev-els of vaginal epithelial terminal differentiation markers Krt6b and Krt13 and the proliferation marker Ki67 were increased after estrogen treatment.Conclusions:A hormone responsive rat vaginal organoid model was successfully constructed using Matrigel embedding and infiltration culture method.关键词
大鼠/阴道/类器官/模型建立及鉴定/激素Key words
Rat/Vagina/Organoid/Establishment and identification/Hormone分类
医药卫生引用本文复制引用
张莉,范琳媛,张心,李婷,张展,宗晓楠,白会会,刘朝晖..一种对激素具有反应性的大鼠阴道类器官模型建立及鉴定[J].现代妇产科进展,2024,33(5):362-368,7.
