一种对激素具有反应性的大鼠阴道类器官模型建立及鉴定OACSTPCD

Objective:To construct a physiologically relevant rat vaginal organoid mod-el responsive to hormone.Methods:Rat vaginal tissues were isolated and disintegrated into cell suspension and coated with Matrigel,then cultured with organoid medium.HE staining and im-munofluorescence were performed to compared organoid with the original tissue in the morpho-logical characteristics and characterize keratin and proliferation marker in vaginal epithelial cells.Estrogen and progesterone treatment were performed to verify the responsiveness of or-ganoid to hormones.Results:The rat vaginal organoids were solid spheres with a diameter of a-bout 100μm.HE staining showed that it was composed of multiple layers of epithelial cells,and immunofluorescence staining showed that the expressions of vaginal epithelial markers such as Krt5/8,Krt15,Ki67 and TP63 were basically consistent with the original tissue.The mRNA lev-els of vaginal epithelial terminal differentiation markers Krt6b and Krt13 and the proliferation marker Ki67 were increased after estrogen treatment.Conclusions:A hormone responsive rat vaginal organoid model was successfully constructed using Matrigel embedding and infiltration culture method.