芪苈强心胶囊联合沙库巴曲缬沙坦对心力衰竭大鼠的作用研究OACSTPCD

[Objective]To observe the effect of Qili Qiangxin Capsule(QLQX)combined with sacubitril valsartan sodium tablets(LCZ696)on the rat model of heart failure.[Methods]Selecting forty adult male SD rats,eight rats were randomly selected individuals as the control group,the remaining 32 rats developed a heart failure model by intraperitoneal injection for 6 weeks,the control group received an intraperitoneal injection of an equivalent amount of normal saline.After successfully establishmenting the animal model,32 rats were randomly divided into QLQX combined with LCZ696,QLQX,LCZ696 and model groups,8 animals in each group,Gavage for 4 weeks.The cardiac structure and function of the rats were evaluated by colour Doppler echocardiography before and after the administration,obtained transthoracic 2D M echocardiography,and detect ejection fraction(LVEF),short axis shortening rate(LVFS),left ventricular end diastolic diameter(LVEDD),left ventricular end systolic diameter(LVESD),left ventricular posterior end diastolic wall thickness(LVPWD),diastolic ventricular septal thickness(IVSD),left ventricular end systolic volume(LVESV)and other parameters;cardiac histomorphological changes were observed by HE staining;Masson Cardiac fibrosis in each group was observed by trichrome staining;enzyme-linked immunoassay is used to determine the levels of N-encephalic natriuretic peptide precursor(NT-proBNP),the index of RAAS system and inflammatory factors.[Results]Compared with the model group,all three treatment groups could reduce the fatigue,poor appetite,loose stool,edema in rats with heart failure to some extent,however,the QLQX combined with the LCZ696 group of rats had the best effect;the whole heart mass index of rats in the three treatment groups improved obviously(P＜0.05),however,there was no significant difference between the three groups(P＞0.05);LVEF and LVFS were significantly increased(P＜0.05),and LVEDD,LVESD,LVESV,LVPWD,and IVSD were all significantly decreased(P＜0.05);compared with QLQX group and the LCZ696 group,LVEF and LVFS were increased significantly in QLQX combined with LCZ696 group(P＜0.01),and LVEDD,LVESD,LVESV,and LVPWD were significantly decreased(P＜0.05);no significant difference in IVSD between the three treatment groups(P＞0.05);compared with the model group,the levels of NT-proBNP,RAAS system indicators and inflammatory factors were significantly reduced in the three treatment groups(P＜0.05),the degree of cardiomyocyte injury was significantly reduced,and the degree of myocardial fibrosis was significantly improved(P＜0.05);QLQX and LCZ696 group performed better than the other two groups(P＜0.05).[Conclusion]QLQX combined with LCZ696 can effectively improve the general state of heart failure rats,improve ventricular remodeling and myocardial remodeling.It can effectively inhibit the RAAS system and reduce the level of inflammatory factors,and is more efficient than QLQX or LCZ696 alone.