食品与发酵工业2024，Vol.50Issue(9)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.036439
灭活卷曲乳杆菌CCFM1118缓解幽门螺杆菌感染的作用评价
Evaluation of the effect of inactivated Lactobacillus crispatus CCFM1118 against Helicobacter pylori infection
摘要
Abstract
Helicobacter pylori(H.pylori)is a common pathogenic bacterium in humans,which is widely spread in the world.It is a pathogen with potential health hazards associated with a variety of diseases,especially digestive diseases such as chronic gastritis and stomach cancer.Antibiotics are commonly used to treat H.pylori infection,but this has many side effects.In recent years,probiotics and their products have been used for the treatment of H.pylori infection due to their safety,low side effects,and effective elimination.This study aimed to evaluate the alleviating effect of postbiotic element CCFM1118-Inactivated Bacteria(CCFM1118-IB)prepared from Lacto-bacillus crispatus CCFM1118 on H.pylori infection.The effect of CCFM1118-IB on H.pylori growth and colonization was evaluated using the antibacterial experiment,cell adhesion assay,and strain coaggregation assay.Meanwhile,55 patients with H.pylori infection were treated with CCFM1118-IB at a dose of 2 g twice daily for one month.13 C-urea breath test,serum biochemical parameters,serum pepsino-gen concentrations,serum cytokine concentrations,and the gut microbiota of patients were measured at baseline and end-of-trial to analyze the effect of CCFM1118-IB in eradicating H.pylori infection in patients.In vitro study results showed that CCFM1118-IB formed an inhibi-tion circle with(12.25±0.29)mm and had a co-aggregating effect with H.pylori,but it was unable to inhibit the adhesion of H.pylori to cells.In the clinical trial,CCFM1118-IB significantly reduced the 13C-Urea breath test value,inhibited the elevation of PG Ⅰ/PG Ⅱ,and affected the abundance of gut microbiota such as Bacteroides and Ruminococcus in patients.Further analysis found that the regulation of the gut microbiota by CCFM1118-IB may promote the production of beneficial active metabolites,thereby improving the health of the host.However,CCFM1118-IB did not significantly affect the general serum biochemistry and other indicators,indicating that it does not affect the normal physiological status of the subjects.The results demonstrated that CCFM1118-IB has anti-H.pylori properties and diminish the harmful effects of H.pylori infection on human health.关键词
益生菌/卷曲乳杆菌/后生元/幽门螺杆菌/临床试验Key words
probiotics/Lactobacillus crispatus/postbiotics/Helicobacter pylori/clinical trial引用本文复制引用
陈峰,李敏玉,刘玲,于雷雷,耿芊,田丰伟,翟齐啸..灭活卷曲乳杆菌CCFM1118缓解幽门螺杆菌感染的作用评价[J].食品与发酵工业,2024,50(9):1-8,8.基金项目
江苏省自然科学基金优秀青年基金项目(BK20220155) （BK20220155）
江苏省重点研发计划(社会发展)项目(BE2021623) （社会发展）
