食品与发酵工业2024，Vol.50Issue(9)：36-42,7.DOI:10.13995/j.cnki.11-1802/ts.035954
转录组学分析空气对泡菜"生花"酵母菌的影响机制
Transcriptomics-based mechanism of air influence on flower-producing yeast in pickles
摘要
Abstract
The proper sealing of the pickle jar/pond is a crucial factor that impacts the blooming of pickles.To elucidate the impact of air on yeast flowering,transcriptomics and bioinformatics were employed to scrutinize and unravel pivotal genes that modulate yeast flores-cence.A total of 183 differentially expressed genes were identified in the flower-producing yeast cultured with or without air,including 42 up-regulated genes and 141 down-regulated genes(P＜0.05).The differentially expressed genes were predominantly enriched in glucosi-dase activity,arabinose dehydrogenase activity,and ribosomal subunit.The differentially expressed genes were found to be enriched in 34 pathways.Among them,the ribosome pathway was identified as the most significant one,with 19 up-regulated genes such as RPL4,RPL10 and RPSA,and 2 down-regulated ones including RPS2 and RPS15.In addition,FAD2 was up-regulated in both fatty acid synthesis pathway and unsaturated fatty acid synthesis pathway while 6 genes(FAS1,FAS2 and SCD etc.)were down-regulated.The qRT-PCR re-sults demonstrated that the expression levels of pivotal genes,namely FAD2,RPL4 and RPL10,were significantly up-regulated in air cul-ture(P＜0.05),which was consistent with the transcriptome-based predictions.The findings establish a theoretical foundation for under-standing the flowering mechanism of yeast in pickles and optimizing pickle quality control.关键词
泡菜/"生花"酵母菌/转录组学/品质控制/空气Key words
pickles/flowering yeast/transcriptomics/quality control/air引用本文复制引用
罗思洋,练银银,杨宇航,谭兆涛,潘玉龙,索化夷,宋佳佳,张玉..转录组学分析空气对泡菜"生花"酵母菌的影响机制[J].食品与发酵工业,2024,50(9):36-42,7.基金项目
重庆市技术创新与应用发展专项(CSTB2022TIAD-XCZXX0025,CSTB2023TIAD-KPX0028) （CSTB2022TIAD-XCZXX0025,CSTB2023TIAD-KPX0028）
创新创业训练计划项目(X202310635132) （X202310635132）
