食品与发酵工业2024，Vol.50Issue(9)：78-83,6.DOI:10.13995/j.cnki.11-1802/ts.035457
三种单萜对皿式培养樟芝三萜合成的影响
Effect of three monoterpenoids on triterpenoids production of Antrodia cinnamomea in petri-dish culture
摘要
Abstract
Antrodia cinnamomea is a kind of edible medicinal fungus.Triterpenoids are one of the dominant active ingredients in the fruiting bodies of A.cinnamomea,which have pharmacological effects such as anti-cancer,hepatoprotective and anti-inflammatory.Wild fruiting bodies of A.cinnamomea are only found on the inner rotten wall of Cinnamomum kanehirae,which leads to the fact that the active products of A.cinnamomea cannot satisfy the huge market demand.Petri-dish culture is a method for artificial cultivation of A.cinna-momea,which has the advantages of short cultivation time and simple operation.But the triterpenoids production of the A.cinnamomea mycelia in petri-dish culture is low.C.kanehirae is the only wild host of A.cinnamomea.Previous studies had indicated that extracts of C.kanehirae could promote the growth and triterpenoids production of mycelia in petri-dish culture.However,there are few reports on the effects of a single substance from C.kanehirae on triterpenoids synthesis of A.cinnamomea in petri-dish culture.To enhance the tirterpe-noids production of A.cinnamomea mycelia,three monoterpenoids(α-terpineol,terpinene-4-ol and α-terpinene)from C.kanehirae were added in the medium in petri-dish culture.Biomass and triterpenoids yield of the mycelia were used as detection indices.The results indi-cated that α-terpineol was the only one among the three monoterpenoids had the ability to promote the growth of A.cinnamomea mycelia in petri-dish culture.The optimal adding concentration of α-terpineol was 0.08 g/L and the biomass of A.cinnamomea mycelia reached to 165.67 mg/plate.Three monoterpenoids all have the promotion effect on synthesis of triterpenoids in A.cinnamomea in petri-dish culture.The optimal adding concentration of α-terpineol is 0.08 g/L and the triterpenoids yield of A.cinnamomea mycelia reached to 17.55 mg/g with 50.39％increase compared with the control group,in the meanwhile,one triterpenoid,dehydrosulphurenic acid,was detected 3.20-fold increase compared with the control group.The optimal adding concentration of terpinene-4-ol is 0.12 g/L and the triterpenoids yield of A.cinnamomea mycelia reached to 8.89 mg/g with 28.65％increase compared with the control group.The optimal adding concentration of α-terpinene is 0.16 g/L and the triterpenoids yield of A.cinnamomea mycelia reached to 13.56 mg/g with 55.68％increase compared with the control group,in the meanwhile,two triterpenoids,dehydrosulphurenic acid and dehydroeburicoic acid,were detected 5.56-fold and 0.96-fold increase respectively compared with the control group.In this study,three monoterpenoids that could promote triterpenoids production of A.cinnamomea in petri-dish culture were identified and it provides new ideas for promoting triterpenoids synthesis of A.cin-namomea in petri-dish culture.关键词
樟芝/三萜/皿式培养/α-松油醇/松油烯/4-萜烯醇/去氢硫色多孔菌酸/去氢齿孔菌酸Key words
Antrodia cinnamomea/triterpenoids/petri-dish culture/α-terpineol/α-terpinene/terpinene-4-ol/dehydrosulphurenic acid/de-hydroeburicoic acid引用本文复制引用
李心阳,罗志珊,耿燕,任怡琳,许泓瑜,史劲松,陆震鸣,许正宏..三种单萜对皿式培养樟芝三萜合成的影响[J].食品与发酵工业,2024,50(9):78-83,6.基金项目
国家自然科学基金项目(31970061) （31970061）
