食品与发酵工业2024，Vol.50Issue(9)：106-116,11.DOI:10.13995/j.cnki.11-1802/ts.035684
AB-8树脂联用C18柱分离牡丹籽粕中新橙皮苷及生物活性研究
Separation of neohesperidin from peony seed meal by AB-8 resin-C18 column coupling and biological activity study
摘要
Abstract
In this study,neohesperidin in peony seed meal was isolated and purified by AB-8 resin combined with C18,and its bio-logical activity was explored,which provided a basis for the development of related products.Six macroporous resins(AB-8,X-5,DM301,CAD-40,D101,and NKA)were screened by the adsorption kinetics model,and the adsorption/analytical capacity of AB-8 res-in was superior to the other five.The optimal separation conditions for AB-8 were that the loading rate was 8 BV/h,the loading volume was 60 mL,the eluent was 60％(volume fraction)ethanol-aqueous solution,and the elution flow rate was 5 mL/min.The antioxidant activity of 1 mg/mL neohesperidin was 83.23％,81.05％,and 72.03％for DPPH free radicals,·O2-,and ABTScationic radicals,respec-tively.250 mg/mL of new hesperidin can improve the antioxidant activity in the cell activity to reduce intracellular reactive oxygen species content.Experiments showed that the inhibition rate of 2 000 mg/mL of new hesperidin on K562 was 92.49％,and 500 mg/mL of new hesperidin could effectively inhibit the K562 G1 phase of division.Molecular dynamics simulation results showed that neohesperidin inhibi-ted the proliferation of K562 through hydrogen bonding with B-cell lymphoma-2 and cyclin-dependent kinase 2.In this study,the active substance neohesperidin was isolated from peony seed meal,which provided a theoretical basis for scientific understanding of peony seed meal waste.关键词
新橙皮苷/大孔树脂/生物活性/分子动力Key words
neohesperidin/macroporous resin/biological activity/molecular dynamics引用本文复制引用
邹平,陈文涛,胡建刚,徐莹,张迎阳,孙承骏,夏炜芳,高蕙文..AB-8树脂联用C18柱分离牡丹籽粕中新橙皮苷及生物活性研究[J].食品与发酵工业,2024,50(9):106-116,11.基金项目
常州市科技项目(CE20212030) （CE20212030）
浙江省市场监督管理局科技立项计划项目(20210157) （20210157）
常州市科技支撑计划项目(20210157) （20210157）
