食品与发酵工业2024，Vol.50Issue(9)：148-156,9.DOI:10.13995/j.cnki.11-1802/ts.035439
枳椇子多糖的酸提取工艺优化及其理化性质与抗氧化活性研究
Optimization of acid extraction process for polysaccharide from Hovenia dulcis seed and analysis of its antioxidant activity
摘要
Abstract
This study aimed to optimize the extraction process of Hovenia dulcis polysaccharide and investigate its physicochemical properties and antioxidant activity.The crude polysaccharide of H.dulcis was extracted by acid solvent,and the extraction process parame-ters were optimized by single-factor investigation and orthogonal experiment.The polysaccharide of H.dulcis was separated and purified by the Sevag method and DEAE-52 anion exchange column,the physical and chemical characteristics were analyzed using high-performance liquid chromatography,high-performance gel permeation chromatography,ultraviolet spectrum,Fourier transform infrared spectrometer,Congo red test,potassium iodide,and scanning electron microscope.The antioxidant activity of H.dulcis polysaccharides was further ana-lyzed.Experimental results indicated that the most quantity of polysaccharide extracted from H.dulcis reached up to 4.07％,in the condi-tions of the solid-liquid ratio being 1∶100,90 ℃ of the extraction temperature,150 min of the extraction time.After separation and purifi-cation,this study obtained a homogeneous refined polysaccharide from H.dulcis,named HDP with a molecular weight of 3.030 x 105 Da,which was a pyran ring acidic polysaccharide with many branches.HDP was composed of mannose,glucosamine,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,rhamnose,and arabinose,with molar percentages of 10.458％,1.442％,8.187％,9.788％,4.536％,4.606％,38.024％,9.948％,and 13.009％.In addition,HDP exhibited a lamellar micromorphology.It was also found that the IC50 values of HDP for scavenging ABTS cationic radicals,DPPH free radicals,and hydroxyl radicals were 0.867 mg/mL,1.178 mg/mL,and 2.112 mg/mL,respectively.Therefore,this study obtained a refined acidic polysaccharide HDP by optimizing the acid extraction process of H.dulcis polysaccharides and revealed that HDP had potential antioxidant activity.The results of this study could provide a theoretical basis for the development of functional food of H.dulcis polysaccharides.关键词
枳椇子多糖/酸提取法/理化性质/抗氧化活性Key words
Hovenia dulcis polysaccharide/acid extraction method/physicochemical property/antioxidant activity引用本文复制引用
刘春阳,白金波,杨尚青,史进阳,秦亚敏,吴德玲,解松子..枳椇子多糖的酸提取工艺优化及其理化性质与抗氧化活性研究[J].食品与发酵工业,2024,50(9):148-156,9.基金项目
国家自然科学基金青年科学基金项目(32001694) （32001694）
安徽省自然科学基金青年项目(2008085QH393) （2008085QH393）
校级科学研究重点项目(2020zrzd14) （2020zrzd14）
高层次人才支持计划项目(2019rczd002) （2019rczd002）
