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苗期喷施茉莉酸甲酯对香稻品种象牙香占香气的影响OA北大核心CSTPCD

Effects of Spraying Methyl Jasmonate at Seedling Stage on Aroma of Fragrant Rice Xiangyaxiangzhan

中文摘要英文摘要

茉莉酸甲酯能够提高植物的抗逆境胁迫能力,但在对香稻香气物质2-乙酰基-1-吡咯啉(2-AP)的影响研究还鲜有报道.以香稻品种象牙香占为试验材料,设置茉莉酸甲酯3种喷施浓度(0.25、0.50和1.00 mmol/L),研究苗期喷施对香稻幼苗2-AP合成与累积、香气前体物质含量、香气相关酶活性、香气相关基因表达量调控、抗性酶活性变化和秧苗素质的影响.结果表明,与对照(CK)相比,喷施适当的茉莉酸甲酯浓度(0.50~1.00 mmol/L),香稻幼苗2-AP含量增加,香气合成前体物质1-吡咯啉、丙酮醛和1-吡咯啉-5-羧酸(P5C)含量提高,二胺氧化酶2基因(DAO4)、鸟氨酸转氨酶基因(OAT)和脯氨酸脱氢酶基因(ProDH)表达量提升.茉莉酸甲酯处理还提高了香稻秧苗素质,增加了抗性酶活性,提高了抗氧化能力.适度的茉莉酸甲酯能提高香稻2-AP含量和秧苗素质,归因于相关酶活性和基因表达量的提高.

Methyl jasmonate is known to enhance plant stress resistance,however,its specific effects on the accumulation of 2-acetyl-1-pyrroline(2-AP)in fragrant rice have not been extensively investigated.In this experiment,Xiangyaxiangzhan,a fragrant rice variety,was selected as the test material.Three concentrations of methyl jasmonate(0.25,0.50,and 1.00 mmol/L)were applied during the seedling stage to examine the impact on the synthesis and accumulation of 2-AP,as well as the levels of aroma precursors,activity of aroma-related enzymes,regulation of aroma-related gene expression,changes in enzyme activities related to stress resistance,and seedling quality.The results showed that compared to the control(CK),spraying appropriate concentrations of methyl jasmonate(0.50-1.00 mmol/L)increased expression of DAO4,OA T,and ProDH in fragrant rice seedlings,accompanied by an elevation in 2-AP content.Furthermore,spraying methyl jasmonate increased the levels of aroma synthesis precursors including 1-pyrroline,methylglyoxal,and P5C.This resulted in an improvement in the quality of fragrant rice seedlings,an increase in the activity of stress-resistant enzymes,and enhanced antioxidant capacity.

邢丕鹏;段美洋;祁剑英;唐湘如;何珍珍;张震;罗昊文;张莹莹;邓思呈;莫钊文;潘圣刚;田华

华南农业大学亚热带农业生物资源保护与利用国家重点实验室/农业农村部华南地区作物栽培科学观测实验站/广州市丝苗香米科学与技术重点实验室,广州 510642

农业科学

香稻2-乙酰基-1-吡咯啉茉莉酸甲酯秧苗素质

fragrant rice2-acetyl-1-pyrrolinemethyl jasmonateseedling quality

《中国稻米》 2024 (003)

67-71,77 / 6

国家自然科学基金(31971843);广东省现代农业产业技术体系(2020KJ105);广州市科技计划项目(202103000075)

10.3969/j.issn.1006-8082.2024.03.011

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