甘草次酸抗弓形虫效果的评价及作用机制研究OA北大核心CSTPCD
Evaluation of the effect and mechanism of glycyrrhetinic acid against Toxoplasma gondii
药物是目前防控弓形虫病的主要手段,筛选新的抗弓形虫药物意义重大.本试验评估了甘草次酸抗弓形虫的效果并初步探究了其作用机制.首先在HFF细胞上测定甘草次酸的CC50,接着通过噬斑试验测定其有无抗弓形虫的效果,综合评估甘草次酸对速殖子入侵宿主细胞和在细胞内增殖的影响,最后采用JC-1 荧光探针和多功能酶标仪分别检测弓形虫线粒体膜电位和活性氧(ROS)的变化情况.结果显示,甘草次酸的CC50 为 351.2 μmol/L.抗弓形虫的IC50 为 28.02 μmol/L,在较高浓度下能抑制弓形虫速殖子在细胞内增殖,甘草次酸发挥抗弓形虫的作用依赖于降低弓形虫线粒体膜电位水平以及刺激活性氧的产生.综上,甘草次酸具有抗弓形虫的效果,具有作为抗弓形虫候选药物的潜力,值得进一步研究.
Toxoplasmosis are mainly controlled by drugs,so screening for new anti-toxoplasmosis drugs is of great important.This study evaluated the anti-T.gondii effect and potential mechanism of glycyrrhetinic acid(GA)in vitro.The CC50 of GA was evaluated on HFF cells using MTT assay.The IC50 of GA were determined by plaque assay,and the influence of glycyrrhetinic acid on tachyzoite invasion and intracellular proliferation were evaluated.The mitochondrial membrane potential and reactive oxygen species(ROS)of T.gondii were detected by the JC-1 probe and automatic microplate reader,respectively.The results showed that CC50 of GA is 351.2 μmol/L,and IC50 of GA was 28.02 μmol/L.It can inhibit T.gondii intracellular proliferation at higher concentrations by decreasing T.gondii mitochondrial membrane potential level and increasing ROS production.In conclusion,GA has the properties of anti-T.gondii,and has potential as anti-Toxoplasma candidate drug,which is worthy of further study.
段银燕;徐琼;樊世杰;黄思扬
扬州大学 兽医学院 比较医学研究院 江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏 扬州 225009扬州大学 兽医学院 比较医学研究院 江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏 扬州 225009||教育部农业与农产品安全国际合作联合实验室,江苏 扬州 25009
畜牧业
弓形虫甘草次酸线粒体机制
Toxoplasma gondiiglycyrrhetinic acidmitochondriamechanism
《中国兽医科学》 2024 (005)
689-695 / 7
江苏省杰出青年基金项目(BK20190046);江苏高校优势学科建设工程资助项目
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