中国兽医科学2024，Vol.54Issue(5)：696-701,6.DOI:10.16656/j.issn.1673-4696.2024.0079
几种功能性物质抑制产肠毒性大肠杆菌的效果及作用机制研究
Inhibitory effect and mechanism of several functional substances on enterotoxic Escherichia coli
摘要
Abstract
IPEC-1 cells were treated with different concentrations of puerarin(PR),ethyl pyruvate(EP),tyrosol(TS)or zinc oxide(ZnO),and then cell viability was detected by CCK-8 method.The lactate dehydrogenase(LDH)kit was used to detect the effect of functional substances on the cytotoxic effects of ETEC K88.Real-time PCR was used to detect the effects of different functional substances on the rel-ative expression levels of genes related to IPEC-1 cells infected with ETEC K88.Results ∶PR,EP and ZnO could promote the proliferation of IPEC-1 cells,and the optimal concentrations were 5 μM,1 mM and 1 μM,respectively.TS had no obvious effect on the proliferation of IPEC-1 cells.After being infected with ETEC K88,the LDH activity of IPEC-1 cells significantly increased(P＜0.05).Compared with the K88 infected group,the addition of 5 μM PR,1 mM EP and 1 μM ZnO significantly reduced LDH activity(P＜0.05).Infection with ETEC K88 caused significant increases in the relative expression of inter-leukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α),chemokine ligand 2(CXCL2),heat shock protein 70(HSP70)and nuclear factor E2-related factor(Nrf2)genes(P＜0.05).The relative expression of cyclin protein D(cyclin D)and villin protein(villin)genes was significantly decreased(P＜0.05).PR significantly increased the relative expression of IL-8 and villin genes in IPEC-1 cells(P＜0.05).EP significantly down-regulated the relative expression of TNF-α and CXCL2 genes in cells(P＜0.05).ZnO significantly increased the relative expression of villin and cyclin D genes(P＜0.05).In addition,PR,EP and ZnO significantly reduced the relative expression of 16S rRNA gene in E.coli genes(P＜0.05).In conclusion,PR,EP and ZnO can inhibit the proliferation of ETEC K88 and have antibacterial effects,EP can reduce the expression of inflammatory genes caused by K88,PR can increase the expression of intestinal villi genes,and ZnO can increase the expression of cell cycle genes.关键词
功能性物质/猪小肠上皮细胞/产肠毒性大肠杆菌Key words
functional substances/porcine small intestinal epithelial cells/ETEC分类
农业科技引用本文复制引用
黄一智,宋转,童庆芳,张艺,王露,李中华,吴涛,侯永清..几种功能性物质抑制产肠毒性大肠杆菌的效果及作用机制研究[J].中国兽医科学,2024,54(5):696-701,6.基金项目
国家自然科学基金区域联合创新发展基金项目(仔猪肠道损伤的营养修复机制研究,U22A20514,2022) （仔猪肠道损伤的营养修复机制研究,U22A20514,2022）
武汉轻工大学动物营养与饲料科学湖北省重点实验室开放课题(202303) （202303）
