北京地区食源性沙门菌消毒剂抗性基因和耐药基因分布及相关性分析OA北大核心
Distribution and Correlation Analysis Between Disinfectant Resistance Genes and Antibiotic Resistance Genes of Foodborne Salmonella spp.in Beijing
为了解北京地区消毒剂抗性基因分布、携带情况及其与耐药基因之间的关系,本试验采集北京市6 个行政区共计39 家超市及农贸市场的猪肉和鸡肉样品,分离培养沙门菌并进行鉴定,随后结合Seqsero2、Staramr和Diamond等生物信息学软件对沙门菌的消毒剂抗性基因和耐药基因进行筛选与分析.结果显示,从 28 家超市和 11 家农贸市场的 148 份猪源和147 份鸡源样品中共分离得到79 株(26.78%)沙门菌.所有分离菌株共预测得到17 种血清型,其中71 株鉴定得到16 种序列型(ST),大部分分离菌株的血清型与ST型一一对应,其中优势菌株为肠炎沙门菌ST11(n =24).共检出 32 个消毒剂抗性基因,其中非共同携带的抗性基因有 5 个,包括外膜孔道蛋白基因opmD/npmC(携带率 72.15%)、有机汞调控蛋白基因merR2(携带率37.97%)、季铵盐类消毒剂抗性基因qacEΔ1(携带率 21.52%)和qacF(携带率 12.66%)、外排泵基因oqxAB(携带率1.27%).共检测出50 个耐药基因,覆盖氨基糖苷类、大环内酯类和四环素类等共10 类抗菌药物,其中氨基糖苷类占比最高(36.00%).相关性分析结果显示,消毒剂抗性基因个数与耐药基因种类数量之间存在显著的正相关(R =0.43,P =6.3×10-5),提示沙门菌在携带消毒剂抗性基因的同时,可能也携带更多种类的耐药基因.本试验为指导食品消毒剂的合理使用、保障北京地区食品安全和疾病预防提供理论支持,具有公共卫生学意义.
To understand the distribution and carriage status of disinfectant resistance genes and their relationship with antibiotic resistance genes in Beijing,this study collected swine and chicken samples from 39 supermarkets and farmer's markets in 6 administrative districts of Beijing,isolated and cultured Salmonella,and then screened and analyzed disinfectant resistance genes and antibiotic resistance genes of Salmonella using bioinformatics software such as Seqsero2,Staramr,and Diamond.The results showed that a total of 79 strains(26.78%)of Salmonella were isolated from 148 swine samples and 147 chicken samples from 28 supermarkets and 11 farmer's markets.A total of 17 serotypes were predicted for all isolated strains,among which 16 sequence types(STs)were identified for 71 strains.The serotypes of most isolates corresponded to ST types one by one,and the dominant strain was Salmonella enteritidis ST11(n =24).A total of 32 disinfectant resistance genes were detected,including 5 non-co-carried resistance genes,including outer membrane porin genes opmD/npmC(carriage rate 72.15%),organic mercury-regulating protein gene merR2(carriage rate 37.97%),quaternary ammonium disinfectant resistance genes qacEΔ1(carriage rate 21.52%)and qacF(carriage rate 12.66%),and efflux pump gene oqxAB(carriage rate 1.27%).A total of 50 antibiotic resistance genes were detected,covering 10 classes of antibiotics such as aminoglycosides,macrolides,and tetracyclines,among which aminoglycosides had the highest proportion(36.00%).The results of correlation analysis showed a significant positive correlation between the number of disinfectant resistance genes and the number of antibiotic resistance gene categories(R =0.43,P =6.3×10-5),suggesting that Salmonella may carry more types of antibiotic resistance genes while carrying disinfectant resistance genes.This study provides theoretical support for guiding the rational use of food disinfectants,ensuring food safety,and disease prevention in Beijing,with public health significance.
马征;翟卫帅;邹之宇;吴聪明;沈建忠;汪洋;刘德俊
中国农业大学动物医学院 兽医公共卫生安全全国重点实验室,北京 海淀 100193
畜牧业
沙门菌全基因组测序消毒剂抗性基因耐药基因
Salmonellawhole-genome sequencingdisinfectant resistance geneantibiotic resistance gene
《中国兽医杂志》 2024 (005)
12-20 / 9
北京市科技计划课题(Z211100007021009);国家自然科学基金专项项目(32141002);国家自然科学基金面上项目(32072922)
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