中国兽医杂志2024，Vol.60Issue(5)：12-20,9.DOI:10.20157/j.cnki.zgsyzz.2024.05.002
北京地区食源性沙门菌消毒剂抗性基因和耐药基因分布及相关性分析
Distribution and Correlation Analysis Between Disinfectant Resistance Genes and Antibiotic Resistance Genes of Foodborne Salmonella spp.in Beijing
摘要
Abstract
To understand the distribution and carriage status of disinfectant resistance genes and their relationship with antibiotic resistance genes in Beijing,this study collected swine and chicken samples from 39 supermarkets and farmer's markets in 6 administrative districts of Beijing,isolated and cultured Salmonella,and then screened and analyzed disinfectant resistance genes and antibiotic resistance genes of Salmonella using bioinformatics software such as Seqsero2,Staramr,and Diamond.The results showed that a total of 79 strains(26.78%)of Salmonella were isolated from 148 swine samples and 147 chicken samples from 28 supermarkets and 11 farmer's markets.A total of 17 serotypes were predicted for all isolated strains,among which 16 sequence types(STs)were identified for 71 strains.The serotypes of most isolates corresponded to ST types one by one,and the dominant strain was Salmonella enteritidis ST11(n =24).A total of 32 disinfectant resistance genes were detected,including 5 non-co-carried resistance genes,including outer membrane porin genes opmD/npmC(carriage rate 72.15%),organic mercury-regulating protein gene merR2(carriage rate 37.97%),quaternary ammonium disinfectant resistance genes qacEΔ1(carriage rate 21.52%)and qacF(carriage rate 12.66%),and efflux pump gene oqxAB(carriage rate 1.27%).A total of 50 antibiotic resistance genes were detected,covering 10 classes of antibiotics such as aminoglycosides,macrolides,and tetracyclines,among which aminoglycosides had the highest proportion(36.00%).The results of correlation analysis showed a significant positive correlation between the number of disinfectant resistance genes and the number of antibiotic resistance gene categories(R =0.43,P =6.3×10-5),suggesting that Salmonella may carry more types of antibiotic resistance genes while carrying disinfectant resistance genes.This study provides theoretical support for guiding the rational use of food disinfectants,ensuring food safety,and disease prevention in Beijing,with public health significance.关键词
沙门菌/全基因组测序/消毒剂抗性基因/耐药基因Key words
Salmonella/whole-genome sequencing/disinfectant resistance gene/antibiotic resistance gene分类
畜牧业引用本文复制引用
马征,翟卫帅,邹之宇,吴聪明,沈建忠,汪洋,刘德俊..北京地区食源性沙门菌消毒剂抗性基因和耐药基因分布及相关性分析[J].中国兽医杂志,2024,60(5):12-20,9.基金项目
北京市科技计划课题(Z211100007021009) （Z211100007021009）
国家自然科学基金专项项目(32141002) （32141002）
国家自然科学基金面上项目(32072922) （32072922）
