中国兽医杂志2024，Vol.60Issue(5)：101-106,6.DOI:10.20157/j.cnki.zgsyzz.2024.05.013
邻近标记技术在弓形虫蛋白质组学研究中的应用进展
Application Advances of Proximity Labeling Technology in Toxoplasma gondii Proteomics Research
摘要
Abstract
Toxoplasma gondii(T.gondii)encodes numerous secretory proteins stored in organelles such as micronemes,rhoptries,and dense granules,which are secreted to different locations before and after the parasite invades host cells and play crucial roles in the infection,parasitism,and pathogenesis processes.The identification and functional studies of these secretory proteins are hotspots and challenges in the research of T.gondii pathogenesis.Traditional proteomic techniques face difficulties in sample purification and low coverage of proteomic information for T.gondii subcellular organelles,and they exhibit lower sensitivity in detecting transient or weak protein interactions.In recent years,the rapidly advancing proximity labeling technology(PL)has overcome these limitations.PL involves fusing bait proteins with specific catalytic enzymes to subsequently add biotin or biotin phenol to label nearby target proteins for biotinylation.This technology integrates the advantages of highly efficient enzyme-catalyzed reactions,enabling efficient labeling of proteins within specific spatial proximity,and can detect weak protein interactions.It has been widely used in biological research in recent years.This paper elaborates on the application of PL catalyzed by biotin ligase(BirA)and ascorbate acid peroxidase(APEX)in T.gondii proteomics research,introduces the contributions of these two labeling methods in the exploration of T.gondii secretory proteins and protein-protein interaction studies,and provides reference for the study of T.gondii pathogenesis and the prevention and control of toxoplasmosis.关键词
邻近标记技术(PL)/弓形虫/蛋白质组学/生物素连接酶(BirA)/抗坏血酸过氧化物酶(APEX)Key words
proximity labeling technology(PL)/Toxoplasma gondii/proteomics/biotin ligase(BirA)/ascorbate peroxidase(APEX)分类
农业科技引用本文复制引用
潘明,李龙娇,葛层层,黄思扬..邻近标记技术在弓形虫蛋白质组学研究中的应用进展[J].中国兽医杂志,2024,60(5):101-106,6.基金项目
江苏省自然科学基金杰出青年基金项目(BK20190046) （BK20190046）
江苏省自然科学基金青年基金项目(BK20230579) （BK20230579）
江苏省高等学校自然科学研究面上项目(20KJB230006) （20KJB230006）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
