中国现代中药2024，Vol.26Issue(4)：616-624,9.DOI:10.13313/j.issn.1673-4890.20240108004
HS-GC-MS结合ROAV分析不同品种橘红花挥发性成分
Detection of Volatile Components in Flos Citri Grandis from Different Plant Varieties by HS-GC-MS Combined with ROAV
摘要
Abstract
Objective:To systematically compare the volatile components and aroma characteristics of Flos Citri Grandis produced from different plant varieties.Methods:Headspace-gas chromatography-mass spectrometry(HS-GC-MS)was employed to detect the volatile components of 40 batches of Flos Citri Grandis samples produced from four plant varieties("Guangqing""Jiaxiyang""Miye Zhengmao"and"Huanglong Zhengmao"),and the key aroma components were analyzed based on the relative odor activity value(ROAV).The significantly differential components and characteristic aroma components of Flos Citri Grandis from different varieties were screened by multivariate statistical analysis.Results:HS-GC-MS identified 40 volatile components of Flos Citri Grandis from four plant varieties.The volatile components were mainly olefins,alcohols,esters,ketones,and aldehydes,among which limonene,β-myrcene,linalool,and trans-nerolidol were the common components with high content.Eight key aroma components shared by the four varieties were screened out based on ROAV.The difference analysis of volatile components showed that 21 differential markers could be used for variety distinguishing,among which β-pinene and linalool were the characteristic aroma components of"Jiaxiyang"and"Miye Zhengmao",respectively,and benzeneacetaldehyde,trans-nerolidol,and methyl anthranilate were the characteristic aroma components of"Huanglong Zhengmao".Conclusion:We combined HS-GC-MS with ROAV to distinguish different varieties of Flos Citri Grandis and identified the differential characteristic aroma components.This study provides reference for variety distinguishing and comprehensive development and utilization of Flos Citri Grandis resources.关键词
橘红花/顶空-气相色谱-质谱法/相对气味活度值/挥发性成分/品种Key words
Flos Citri Grandis/headspace-gas chromatography-mass spectrometry/relative odor activity value/volatile components/varieties分类
医药卫生引用本文复制引用
杨洁慧,杜佳林,李静文,倪晨,刘伟琼,陈泳琪,詹若挺,严萍..HS-GC-MS结合ROAV分析不同品种橘红花挥发性成分[J].中国现代中药,2024,26(4):616-624,9.基金项目
广东省乡村振兴战略专项(2023KJ148) （2023KJ148）
广州中医药大学2023年校级大学生创新创业训练计划项目(202310572195) （202310572195）
