中国生物防治学报2024，Vol.40Issue(2)：299-309,11.DOI:10.16409/j.cnki.2095-039x.2023.01.027
60Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性
Breeding of Butenyl-spinosyns High Yielding Strain by 60Co-NTG Compound Mutation and Its Insecticidal Activity
摘要
Abstract
In order to obtain stable and high-yield butenyl-spinosyn-producing strains and determine its lethal efficiency against agricultural pests,a high-yield mutant strain ASAGF30A11 with stable genetic traits was obtained by compound mutagenesis of a parent strain Saccharopolyspora pogona ASAGF19 screened from the previous study using 60Co-γ-ray and nitrosoguanidine.The new strain increased yield by 61.26%compared with the parent strain,and the yield variation of 3 generations was less than 10%.The crude butenyl-spinosyn was purified from the fermentation broth of the high-yield strain,and then diluted into the mother liquor with a concentration of 1.5 g/L.The insecticidal activity against Thrips palmi Karny,aphids and Plutella xylostella was investigated and the LC50 was determined to be 1.92 mg/L,1.23 mg/L and 0.27 mg/L,respectively.By comparison,butenyl-spinosyn was of a higher lethal efficiency against T.palmi than spinosyn(LC500.58 mg/L)and spinetoram(LC500.64 mg/L),a higher lethal efficiency against aphids than spinosyn(LC5072.60 mg/L)but equal efficiency with avermectin(LC500.14 mg/L),and a higher lethal efficiency against P.xylostella than spinosyn(LC502.9 mg/L)but lower efficiency than spinetoram(LC500.05 mg/L).This study explored the effect of compound mutagenesis using 60Co-γ-ray and nitrosoguanidine on S.pogona for the first time and measured the laboratory lethal efficiency of butenyl-spinosyn against three agricultural pests,which serves as part of the theoretical basis for the control of agricultural pests with new biological insecticides.关键词
须糖多孢菌/60Co-γ射线/亚硝基胍/复合诱变/丁烯基多杀菌素Key words
Saccharopolyspora pogona/60Co-γ-ray/NTG/combined mutagenesis/butenyl-spinosyns分类
农业科技引用本文复制引用
徐周钦,郭超,李金萍,张逍遥,王珂慧,何志平,刘兴泉,王超..60Co-NTG复合诱变选育丁烯基多杀菌素高产菌株及其杀虫活性[J].中国生物防治学报,2024,40(2):299-309,11.基金项目
国家重点研发计划(2023YFA0914700) （2023YFA0914700）
中央公益性科研院所专项课题(JY2311) （JY2311）
企业合作技术开发项目(H20054-1) （H20054-1）
