基于肠道菌群探讨五味子水提物对非酒精性脂肪肝小鼠的影响OACSTPCD

To study the effects of Schisandra chinensis aqueous extract on intestinal flora in nonalcoholic fatty liver disease(NAFLD)mice,a NAFLD model was established by high-fat diet feeding mice for 16 weeks,and Schisandra chinensis aqueous extract was administered by gavage from week 4.Serum ALT,AST,TC and TG levels were measured after 16 weeks.HE and oil red O staining were used to observe pathological changes of mice liver.Immunofluorescence staining was used to observe expressions of IL-6 and TNF-α in mice liver.High-throughput sequencing was used to analyze the changes of intestinal flora in colonic contents.The results indicated that,compared with the control group,the body mass in the mice group with high-dose Schisandra chinensis aqueous extract was significantly reduced.The liver mass in mice groups with low-dose and high-dose Schisandra chinensis aqueous extract was significantly reduced;ALT,AST,TC and TG in each administration group were significantly reduced;mice liver pathology and inflammation were significantly alleviated;the diversity of intestinal flora and abundance of above-mentioned dominant species were significantly corrected,and changes of flora were correlated with pharmacodynamic indexes.Schisandra chinensis aqueous extract had a definite therapeutic effect on NAFLD mice,which might be associated with its improvement of intestinal flora in NAFLD mice.