中国食品添加剂2024，Vol.35Issue(5)：36-45,10.DOI:10.19804/j.issn1006-2513.2024.5.004
基于肠道菌群探讨五味子水提物对非酒精性脂肪肝小鼠的影响
Effects of Schisandra chinensis aqueous extract on gut microbiota of NAFLD mice
摘要
Abstract
To study the effects of Schisandra chinensis aqueous extract on intestinal flora in nonalcoholic fatty liver disease(NAFLD)mice,a NAFLD model was established by high-fat diet feeding mice for 16 weeks,and Schisandra chinensis aqueous extract was administered by gavage from week 4.Serum ALT,AST,TC and TG levels were measured after 16 weeks.HE and oil red O staining were used to observe pathological changes of mice liver.Immunofluorescence staining was used to observe expressions of IL-6 and TNF-α in mice liver.High-throughput sequencing was used to analyze the changes of intestinal flora in colonic contents.The results indicated that,compared with the control group,the body mass in the mice group with high-dose Schisandra chinensis aqueous extract was significantly reduced.The liver mass in mice groups with low-dose and high-dose Schisandra chinensis aqueous extract was significantly reduced;ALT,AST,TC and TG in each administration group were significantly reduced;mice liver pathology and inflammation were significantly alleviated;the diversity of intestinal flora and abundance of above-mentioned dominant species were significantly corrected,and changes of flora were correlated with pharmacodynamic indexes.Schisandra chinensis aqueous extract had a definite therapeutic effect on NAFLD mice,which might be associated with its improvement of intestinal flora in NAFLD mice.关键词
非酒精性脂肪肝/五味子/水提物/高通量测序/肠道菌群Key words
nonalcoholic fatty liver disease/Schisandra chinensis/aqueous extract/high-throughput sequencing/gut microbiota分类
轻工纺织引用本文复制引用
陈二华,刘翔,杨悦,杨蕊,安欣,朱慧芳..基于肠道菌群探讨五味子水提物对非酒精性脂肪肝小鼠的影响[J].中国食品添加剂,2024,35(5):36-45,10.基金项目
江苏省高职院校教师专业带头人高端研修项目(编号:2023GRFX023) （编号:2023GRFX023）
江苏省大学生创新创业培训计划项目(编号:202214541010Y （）
202214541008Y) （）
