中国食品添加剂2024，Vol.35Issue(5)：114-122,9.DOI:10.19804/j.issn1006-2513.2024.5.012
基于菌藻混合培养合成天然类胡萝卜素的工艺研究及转录组水平分析
Natural carotenoids production using a mixed microbial culture of Rhodotorula glutinis and Chlorella vulgaris and its transcriptome analysis
摘要
Abstract
A mixed culture was used in producing natural carotenoids to overcome the problem of low production rate in single microbe culture.An equal volume of Rhodotorula glutinis and Chlorella vulgaris was inoculated in a 5 L fermentation tank.The feasibility of the mixed culture process of bacteria and algae was studied,the molecular mechanism of synergistic metabolism between R.glutinis and C.vulgaris in the mixed system was analyzed at transcriptional level at specific time.The results showed that the maximum biomass,residual sugar utilization rate and carotenoids yield of mixed culture were 51.7 g/L,83.3%and 29.8 mg/L,respectively.The carotenoids yield of experimental group were 21.5%and 77.2%higher than those of the single culture groups of R.glutinis or C.vulgaris,respectively.Moreover,the mixed culture could obtain seven kinds of carotenoids and proportion of zeaxanthin and astaxanthin was also increased.Samples were taken at 36 h of fermentation,and transcriptome sequencing analysis showed that 85 and 2858 genes were obtained in the experimental and the control groups,respectively.Gene ontology classification and KEGG metabolic pathway analysis indicated that C.vulgaris had dual function of obtaining the nutrition and adjusting proportion of carotenoid species by enhancing upregulation genes related to photosynthesis and carotenoids synthesis.关键词
微生物类胡萝卜素/粘红酵母/小球藻/混合培养/转录组Key words
microbial carotenoids/Rhodotorula glutinis/Chlorella vulgaris/mixed culture/transcriptome分类
轻工纺织引用本文复制引用
张志平,段晨阳,魏湘楠,宋丽丽,魏涛..基于菌藻混合培养合成天然类胡萝卜素的工艺研究及转录组水平分析[J].中国食品添加剂,2024,35(5):114-122,9.基金项目
河南省科技攻关项目(212102310077,232102110150) （212102310077,232102110150）
中原科技创新领军人才项目(224200510017) （224200510017）
