中国食品添加剂2024，Vol.35Issue(5)：280-286,7.DOI:10.19804/j.issn1006-2513.2024.5.031
酸水解快速测定法检测特医食品中多种维生素的含量
Rapid determination of multivitamins in special medical food by acid hydrolysis method
摘要
Abstract
A rapid acid hydrolysis method for the determination of VB1,VB2,VB6,and niacinamide in food for special medical purpose was established.The sample was pretreated by HCl at 70℃for 10 minutes.Then protein was precipitated,and VB1,VB2,VB6,and niacinamide were separated and detected by HPLC and diode array detector at the wavelength of 268 nm.The results showed that four components had a good linear relationship in the range from 0.1 to 50.0 μg/mL.The detection limit range was 0.1～0.3 mg/kg,and quantitative limit range was 0.3～0.9 mg/kg.The recovery rate was 87.4%～93.9%,and the relative standard deviation was 3.0%～5.1%(n=5).This method had high accuracy and sensitivity.It can meet the needs of rapid determination of VB1,VB2,VB6,and niacinamide in special medical food.关键词
酸水解/快速测定/特殊医学用途配方食品/水溶性维生素Key words
acid hydrolysis/rapid determination/food for special medical purpose/water-soluble vitamin分类
轻工纺织引用本文复制引用
陈伟,黄银波,乔勇升,蔡霞,董贵军,胡慧..酸水解快速测定法检测特医食品中多种维生素的含量[J].中国食品添加剂,2024,35(5):280-286,7.基金项目
江苏省市场监管局科技计划项目(苏市监科信[2022]87号) （苏市监科信[2022]87号）
