中国食品添加剂2024，Vol.35Issue(5)：333-346,14.DOI:10.19804/j.issn1006-2513.2024.5.038
乳源外泌体的分泌提取及组学研究进展
Progress of secretion,extraction,and omics research of milk-derived exosomes
摘要
Abstract
Exosomes are nanoscale vesicles secreted by eukaryotic cells into the extracellular space,carrying proteins,nucleic acids,and lipids,serving as carriers for intercellular communication.They are widely present in biological fluids,including exosomes derived from breast milk,which have garnered significant attention due to their unique functional properties.However,due to their small size and low density,the extraction process of exosomes poses numerous challenges.Currently,there is no method that can rapidly,efficiently,and non-invasively extract exosomes with high purity,limiting their yield and purity,thus hindering their application in scientific research and clinical fields.This article provided a comprehensive review of the latest technological advancements in exosome extraction and identification,explored effective methods for inducing the secretion of exosomes from extracellular cells,and delved into the research trends in proteomics,nucleic acidomics,and lipidomics of breast milk-derived exosomes.These studies not only help us in comprehensive understanding of the characteristics of breast milk-derived exosomes but also provide a reference for the use of milk-derived exosomes as an alternative to breast milk in the prevention and treatment of clinical diseases in infants and young children.关键词
milk-derived exosomes/extraction and identification/extracellular cell secretion/multi-omicsKey words
milk-derived exosomes/extraction and identification/extracellular cell secretion/multi-omics分类
轻工纺织引用本文复制引用
党兴芬,任佳慧,张珍珍,姬雅静,魏三娃,张伟,陈伙,陈历俊..乳源外泌体的分泌提取及组学研究进展[J].中国食品添加剂,2024,35(5):333-346,14.基金项目
首农食品集团自立科技项目(SNSPKJ(2022)03) （SNSPKJ(2022）
中央引导地方科技发展资金(桂科ZY22096025) （桂科ZY22096025）
国家重点研发计划(2023YFF1104400) （2023YFF1104400）
家畜产业技术体系北京市创新团队项目 （）
