中药材2024，Vol.47Issue(1)：51-56,6.DOI:10.13863/j.issn1001-4454.2024.01.009
药用蛇及其水提物的DNA微型条形码鉴别研究
Identification of Medicinal Snakes and Water Extracts Based on DNA Mini-Barcode
摘要
Abstract
Objective:To establish a method for identification of medicinal snake and its water extract by DNA mini-barcode.Meth-ods:By comparing the 16S rRNA sequences of medicinal snakes and 11 common adulterants,a mini-barcode of about 220 bp of snake DNA was established,and compared with the 16S rRNA standard barcode,the raw materials and water extracts of medicinal snakes were sequenced and identified.MEGA-X software was used to analyze genetic distance and construct phylogenetic tree by adjacency method(NJ).Results:The amplification rate and identification rate of DNA mini-barcode primers for medicinal snake raw materials and water extracts were 100％.The average intraspecific genetic distances of medicinal snakes and the adulterants were less than the average inter-specific genetic distances,All species had good monophyletic characteristics in the NJ phylogenetic tree,which was consistent with the results of 16S rRNA standard barcode raw material identification.Conclusion:The snake DNA mini-barcode established in this study can effectively realize the species identification of medicinal snake raw materials and water extracts,and provides technical support for ensu-ring the safety and effectiveness of snake raw materials,clinical decoction and formula granules.关键词
乌梢蛇/金钱白花蛇/蕲蛇/水提物/DNA微型条形码/16S rRNAKey words
Zaocys dhumnades(Cantor)/Bungarus multicinctus Blyth/Deinagkistrodon acutus(Güenther)/Water extract/DNA mini-barcode/16S rRNA分类
中医学引用本文复制引用
冼乐尧,罗宇琴,宋叶,魏梅,孙冬梅,李国卫,胡绮萍..药用蛇及其水提物的DNA微型条形码鉴别研究[J].中药材,2024,47(1):51-56,6.基金项目
广东省基础与应用基础研究基金项目(2021A1515110790) （2021A1515110790）
