动物医学进展2024，Vol.45Issue(7)：31-37,7.
广西地区腹泻仔猪呼肠孤病毒的感染及序列分析
Infection and Sequence Analysis of Reovirus in Piglets with Diarrhea in Guangxi
摘要
Abstract
In order to understand the infection of mammalian Reovirus(MRV)in diarrhea piglets in Guan-gxi and major circulating strains,173 samples of diarrhea piglets from 2020 to 2022 were detected by nested RT-PCR.Some of the positive samples were selected to amplify and analyze the S1 gene,so as to further understand the genetic characteristics of the main epidemic strains of MRV in Guangxi.The results showed that the total positive rate of 173 samples from 2020 to 2022 was 18％(32/173),and the positive rates were 29.6％,9.9％and 33％respectively.In this study,a full-length sequence of S1 gene was obtained.Se-quence alignment showed that the sequence had 43.4％-97.9％nucleotide homology and 26.1％-97.8％ami-no acid homology with other MRV S1 gene sequences.Phylogenetic analysis showed that the strain se-quence obtained in this study was MRV3,which belonged to lineage Ⅳ in the same branch as other porcine MRV3 strains ZJ2013 and IND/MZ/3013789/reo.The results of this study indicate that there is a certain degree of MRV infection in pigs in Guangxi,which provides a scientific basis for further prevention and control of MRV epidemic in pigs with diarrhea in Guangxi.关键词
哺乳动物呼肠孤病毒/S1基因/序列分析/感染情况Key words
Mammalian Reovirus(MRV)/detection/S1 gene/sequence analysis/infection situation分类
农业科技引用本文复制引用
隆美金,黄伟坚,欧阳康,陆颖,李茂宁,易春华,廖梦娟,余科辰,覃一峰,陈樱,韦祖樟..广西地区腹泻仔猪呼肠孤病毒的感染及序列分析[J].动物医学进展,2024,45(7):31-37,7.基金项目
广西重点研发计划项目(桂科AB23026082) （桂科AB23026082）
广西自然科学基金项目(2021GXNSFAA196067) （2021GXNSFAA196067）
广西大学巴马产教融合研究院专项项目(巴人科20220015)和广西百人计划项目. （巴人科20220015）
