福建农业学报2024，Vol.39Issue(3)：290-301,12.DOI:10.19303/j.issn.1008-0384.2024.03.006
基于冬凌草全长转录组的TIFY基因家族鉴定与表达分析
Identification and Expressions of TIFY Family Based on the Full-Length Transcriptome in Isodon rubescens
摘要
Abstract
[Objective]TIFY protein is a key regulator of the JA signalling pathway and plays a significant regulatory role in plant growth and development,abiotic stress and the accumulation of secondary metabolites.The identification of the TIFY gene in Isodon rubescens provides a theoretical foundation for the breeding of I.rubescens with enhanced stress tolerance and the investigation of the synthesis of secondary metabolites.[Method]TIFY family was identified using bioinformatic methods based on the full-length transcriptome database of I.rubescens.Expressions of the genes in tissues were analyzed by RT-qPCR.[Result](1)A total of 12 IrTIFYs genes were identified in I.rubescens.(2)The amino acid length was 124-378,the molecular weight 13 924.89-39 692.38 Da,and the isoelectric point ranged from 5.05 to 9.69.All members were unstable proteins,except for IrTIFY10.IrTIFY proteins were all located in the nucleus and were hydrophilic proteins without signal peptides.(3)Structural analysis indicates that IrTIFY proteins lack transmembrane structure and that the most abundant secondary structure type is random coil.Furthermore,all TIFY proteins contain multiple phosphorylation sites.(4)The IrTIFY gene family had weak codon preference,with a slight tendency to use codons ending in A or U.(5)There were many light-,hormone-,and stress-responsive cis-elements in the IrTIFY gene family,but cis-elements were difference in numbers and types among different members.(6)Evolutionary tree analysis showed that the 12 members of the TIFY family were divided into four subfamilies:PPD(IrTIFY2),ZML(IrTIFY3/8/10),TIFY(IrTIFY7/12),and JAZ(IrTIFY1/4/5/6/9/11).They were closest to that of Salvia miltiorrhiza of Labiaceae family.(7)RT-qPCR analysis revealed that the expression of all 12 members of the TIFY family of I.rubescens in different tissues was as follows:leaves>stems>roots,and most of them were significantly different.[Conclusion]Based on the above results,it is hypothesised that the TIFY gene family plays an important regulatory role in the growth and development of I.rubescens and may be involved in the regulation of the synthesis of secondary metabolites of I.rubescens,which lays the foundation for further in-depth study of the function of the TIFY gene family in I.rubescens and provides an idea for the further study of the function of the TIFY gene family in I.rubescens.关键词
冬凌草/TIFY基因家族/生物信息学分析/表达分析Key words
Isodon rubescens/TIFY gene family/bioinformatics analysis/expression analysis分类
医药卫生引用本文复制引用
练从龙,兰金旭,杨晶凡,李晶晶,杨灏,陈随清..基于冬凌草全长转录组的TIFY基因家族鉴定与表达分析[J].福建农业学报,2024,39(3):290-301,12.基金项目
河南省科技研发计划联合基金项目(优势学科培育类)(232301420078) （优势学科培育类）
河南省中药材产业技术体系项目(豫科项[2022]30 号) （豫科项[2022]30 号）
中医药公共卫生服务补助专项(豫财社[2019]40 号) （豫财社[2019]40 号）
