福建农业学报2024，Vol.39Issue(3)：302-309,8.DOI:10.19303/j.issn.1008-0384.2024.03.007
西番莲种质资源花粉活力鉴定方法探究
Determination of Viability of Passion Fruit Pollens
摘要
Abstract
[Objective]Method for determining the viability of passion fruit pollens was explored to facilitate the utilization of existing germplasms and breeding new varieties.[Methods]Medium and conditions for in vitro culture of Passiflora pollens was optimized.Appropriate staining methods to determine and storage conditions to maximize the pollen viability were investigated.Contents of sucrose,H3BO3,Ca(NO3)2,and polyethylene glycol-4000(PEG-4000)of the medium were optimized in an orthogonal experiment on pollens of 6 germplasms.Pollen germination on the finalized medium for varied culture times was observed.The staining of I2-KI,TTC,and Alexander methods and storage for different durations at different temperatures were compared.[Results]The optimum medium was formulated with 100 g·L-1 sucrose,0.02 g·L-1 H3BO3,0.04 g·L-1 Ca(NO3)2,150 g·L-1 PEG-4000,200 g·L-1 MgSO4·7H2O,and 100 g·L-1 KNO3,then adjusted to pH 5.5.The pollen germination was examined in 1 h of the in vitro culture.TTC staining showed satisfactory effect with no significant deviations from the in vitro observation indicating it an applicable indicator for pollen viability.In 24 h of storage at 25℃the pollen viability was 30.48%;and at 4℃,it was 26.69%and remained 26.54%-29.05%for 7 d.[Conclusion]The medium formulation and in vitro culture time as well as the staining method and storage conditions of passion fruit pollens were determined making a rapid and reliable procedure to maximize the pollen germination available.关键词
西番莲/花粉活力/花粉培养基/花粉离体萌发/染色法/花粉贮藏Key words
Passion fruit/pollen viability/culture medium/in vitro pollen germination/staining/pollens storage分类
农业科技引用本文复制引用
项艳艳,黄伟杰,赖江龙,汤礼梅,林挺兴,李亮,魏秀清,许家辉..西番莲种质资源花粉活力鉴定方法探究[J].福建农业学报,2024,39(3):302-309,8.基金项目
福建省高质量发展超越"5511"协同创新工程项目(XTCXGC2021006) （XTCXGC2021006）
福建省种业创新与产业化工程项目(zycxny2021010-1) （zycxny2021010-1）
福建省农业科学院科技创新团队建设项目(CXTD2021008-2) （CXTD2021008-2）
福建省科技计划公益类专项(2021R1028002) （2021R1028002）
