西番莲种质资源花粉活力鉴定方法探究OA北大核心CSTPCD

[Objective]Method for determining the viability of passion fruit pollens was explored to facilitate the utilization of existing germplasms and breeding new varieties.[Methods]Medium and conditions for in vitro culture of Passiflora pollens was optimized.Appropriate staining methods to determine and storage conditions to maximize the pollen viability were investigated.Contents of sucrose,H3BO3,Ca(NO3)2,and polyethylene glycol-4000(PEG-4000)of the medium were optimized in an orthogonal experiment on pollens of 6 germplasms.Pollen germination on the finalized medium for varied culture times was observed.The staining of I2-KI,TTC,and Alexander methods and storage for different durations at different temperatures were compared.[Results]The optimum medium was formulated with 100 g·L-1 sucrose,0.02 g·L-1 H3BO3,0.04 g·L-1 Ca(NO3)2,150 g·L-1 PEG-4000,200 g·L-1 MgSO4·7H2O,and 100 g·L-1 KNO3,then adjusted to pH 5.5.The pollen germination was examined in 1 h of the in vitro culture.TTC staining showed satisfactory effect with no significant deviations from the in vitro observation indicating it an applicable indicator for pollen viability.In 24 h of storage at 25℃the pollen viability was 30.48%;and at 4℃,it was 26.69%and remained 26.54%-29.05%for 7 d.[Conclusion]The medium formulation and in vitro culture time as well as the staining method and storage conditions of passion fruit pollens were determined making a rapid and reliable procedure to maximize the pollen germination available.