核农学报2024，Vol.38Issue(7)：1249-1257,9.DOI:10.11869/j.issn.1000-8551.2024.07.1249
西洋参根腐病拮抗菌XT-25的生防作用研究
Study on the Biocontrol Effect of Antagonistic Bacteria XT-25 against Panax quinquefolium Root Rot
摘要
Abstract
This study explored the application of biological control technology in managing the root rot disease of American ginseng.Fusarium solani and Ilyonectria mors-panacis,two major fungal pathogens causing root rot,were used as targets to screen antagonistic biocontrol bacteria from the rhizosphere soil of American ginseng.Through confrontation culture experiments,the antagonistic bacteria were screened based on their mycelial inhibition activity,bacteriostatic activity,effects on promoting biomass,and ability in controlling pathogen growth in the inoculation experiments in vitro.The results showed that a strain,XT-25 was isolated,which has a>55%inhibition rate against two species of American ginseng pathogens.Combining morphological characteristics and phylogenetic analysis of 16S rRNA,atpD and rpoB genes,strain XT-25 was identified as Streptomyces lavendulae.In addition to root rot inducing fungi,XT-25 showed a 59%～70%inhibitory effect against 6 other pathogens such as F.oxysporum f.sp.cucumerinum.Next,the antifungal mechanism of XT-25 on root rot of American ginseng were investigated.Notably,XT-25 has the ability to secrete enzymes such as protease,amylase and β-1,3 glucanase to inhibit the growth of fungi.It has the ability to dissolve organic phosphorus and inorganic phosphorus,produce ammonia,fix nitrogen,and secrete IAA and ACC deaminase,which may contribute to biomass promotion.Meanwhile,the volatile gas constituent of strain XT-25 showed a 9.04%inhibition rate against F.solani.It also induced deformity of F.solani and I.mors-panacis hyphae.For the in vitro American ginseng root inoculation experiments,XT-25 demonstrated 40.00%and 24.74%control effects on the root rot caused by F.solani and I.mors-panacis,respectively.In summary,the study of strain XT-25 can provide biocontrol materials and theoretical references for the prevention and treatment of Panax quinquefolium root rot caused by Fusarium and Ilyonectria.关键词
西洋参根腐病/生防菌/淡紫灰链霉菌/拮抗促生作用Key words
Panax quinquefolium root rot/biocontrol bacteria/Streptomyces lavendulae/antagonistic and plant growth gromoting effect引用本文复制引用
张铭鑫,彭娜,王尧尧,王仪,李宝通,刘慧芹,高微微..西洋参根腐病拮抗菌XT-25的生防作用研究[J].核农学报,2024,38(7):1249-1257,9.基金项目
国家重点研发计划课题(2022YFC3501501),河北省重点研发计划(22347402D),中国医学科学院医学与健康科技创新工程(2021-I2M-1-031),天津市农业科技成果转化与推广项目(202101080) （2022YFC3501501）
