湖南农业大学学报（自然科学版）2024，Vol.50Issue(2)：57-64,8.DOI:10.13331/j.cnki.jhau.2024.02.009
贵州紫皮百香果果腐病病原菌的鉴定及其与各发育阶段真菌微生物群落的关系
Identification of fungal pathogens causing rot disease of purple passion fruit(Passiflora edulis)in Guizhou and its correlation with fungal microbial community at various developmental stages of purple passion fruit
摘要
Abstract
Fungal pathogens causing rot disease of purple passion fruit in Guizhou were isolated,morphology and molecular identification showed 15 pathogen strains belonged to Lasiodiplodia brasiliensis,Fusarium proliferatum,Diaporthe tectonendophytica,Diaporthe unshiuensis,D.tectonae and D.hubeiensis.Pathogenicity study revealed that 1 strain showed weak virulence,4 strains showed moderate virulence,4 strains showed fairly strong virulence,and 6 strains showed strong virulence.Lasiodiplodia brasiliensis strain BXG-Z-1 and D.hubeiensis strains showed strong virulence.Diversity analysis of fungal communities at different periods of development of purple passion fruit showed that fungal community OTUs of purple passion fruit in fruit flowers,young fruits and mature fruits were 26,23 and 20 respectively,while simpson index ranged from 0.095 to 0.113,which indicated low richness of fungal community.Shannon index of all samples was below 0.5,indicating low evenness of fungal community,and correspondingly,relative abundance of Microdium spp.exceeded 90%at different growth period of passion fruit.However fungi with low relatively abundance have significant changes during different growth periods of purple passion fruit:relative abundance of Cladosporium spp.,and Ruinenia spp.in the flower stage of passion fruit were the highest,and decreased to the lowest in young fruit stage,and increased again in mature fruit stage,while relative abundance of Trichoderma spp.,Bipolaris spp.,Amanita spp.,Monilinia spp.and Cercospora spp.in flower stage were significantly higher than that in young fruit stage,and further decreased to the lowest at maturity stage.关键词
紫皮百香果/病原菌/微生物多样性/贵州Key words
purple passion fruit/pathogenic bacteria/microbial diversity/Guizhou分类
农业科技引用本文复制引用
雷霁卿,石银娜,巴良杰,吉宁,王瑞..贵州紫皮百香果果腐病病原菌的鉴定及其与各发育阶段真菌微生物群落的关系[J].湖南农业大学学报（自然科学版）,2024,50(2):57-64,8.基金项目
贵州省创新平台建设计划(黔科中引地[2020]4018号) （黔科中引地[2020]4018号）
贵阳学院研究生科研基金项目(GYU-YJS[2021]-44) （GYU-YJS[2021]-44）
