海洋科学2024，Vol.48Issue(3)：85-94,10.DOI:10.11759/hykx20230926001
盐度对绿鳍马面鲀存活、抗氧化酶及组织结构的影响
Effect of salinity on survival,antioxidant enzymes and tissue structure of Thamnaconus septentrionalis
摘要
Abstract
The greenfin horse-faced filefish(Thamnaconus septentrionalis),an emerging species for cage aquaculture along the coast of China,undergoes significant salinity fluctuations during the rainy season in summer.Therefore,understanding the impact of salinity on the survival and physiological indicators of T.septentrionalis is of great significance for the development of cage aquaculture.In this study,the 96 h semilethal salinity of T.septentrionalis was determined.Subsequently,the low-salinity(15)and high-salinity(40)groups were established.Dynamic changes in physiological indicators after salinity stress at different time points were analyzed,and the changes in the tissue structure of the liver,gills,and kidneys were recorded.The results indicated that the 96-h semilethal salinity was 10.74 for the low-salinity group and 42.95 for the high-salinity group.The Na+-K+-ATPase activity in the gills of both the low-and high-salinity groups indicated an increasing trend,which was significantly higher than that in the control group at 96 h(P<0.05).The activities of superoxide dismutase and catalase in the liver of both the low-and high-salinity groups revealed an initial increase followed by a decreasing trend,and at 96 h,the activities were lower in these groups than in the control group.The activity of glutathione peroxidase in the low-salinity group showed a decreasing trend,followed by an increase,and,at 96 h,it was significantly lower than that in the high-salinity group(P<0.05).Malondialdehyde content in the liver of the low-and high-salinity groups reached a maximum at 96 h,which was significantly higher than that in the control group(P<0.05).The tissue sections revealed that hepatocytes in the low-and high-salinity groups exhibited vacuolation and nuclear condensation,with more severe effects in the high-salinity group.The gill epithelial cells displayed a disorderly arrangement and cell necrosis,with widened filaments and elongated lamellae in the low-salinity group,whereas the high-salinity group exhibited filament shrinkage and increased lamellar spacing.In the kidney tissues,the low-salinity group showed dilation of the renal tubules and enlargement of the renal sacs,whereas the high-salinity group exhibited necrosis of the renal tubules and glomeruli.The findings on the effects of salinity on survival,antioxidant enzymes,and tissue structure in this study provide theoretical support for the healthy aquaculture of T.septentrionalis.关键词
绿鳍马面鲀(Thamnaconus septentrionalis)/盐度/抗氧化/组织结构Key words
Thamnaconus septentrionalis/salinity/antioxidant/organizational structure分类
农业科技引用本文复制引用
张子阳,边力,成永旭,李凤辉,陈四清,常青,陈莹,徐荣静,彭立成,吴丹..盐度对绿鳍马面鲀存活、抗氧化酶及组织结构的影响[J].海洋科学,2024,48(3):85-94,10.基金项目
中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022022014) （20603022022014）
福建省海洋渔业资源与生态环境重点实验室开放基金课题项目(Z822286) （Z822286）
烟台市科技创新发展计划项目(2022XCZX078) （2022XCZX078）
福建省区域发展项目(2022N3015) Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS,No.20603022022014 （2022N3015）
Open Fund Project of Fujian Provincial Key Laboratory of Marine Fishery Resources and Ecological Environment,No.Z822286 （）
Yantai Science and Technology Innovation Development Program Projects,No.2022XCZX078 （）
Regional Develop-ment Project in Fujian Province,No.2022N3015 （）
