吉林农业大学学报2024，Vol.46Issue(2)：211-218,8.DOI:10.13327/j.jjlau.2020.5228
吉林省水稻恶苗病菌对咯菌腈的敏感性及抗药性风险评估
Sensitiveness and Resistance Risk Assessment of Rice Fusarium fuji-kuroi to Fludioxonil in Jilin Province
摘要
Abstract
The study aims to understand the sensitiveness of Fusarium fujikuroi to fludioxonil and as-sess the risk of resistance.From 2016 to 2018,508 strains of F.fujikuroi were isolated from 16 re-gions of Jilin province,and sensitiveness of 136 strains of F.fujikuroi to fludioxonil was determined by mycelial growth rate method.Resistant mutants of rice F.fujikuroi to fludioxonil were obtained by drug domestication.The results showed that EC50 values of 136 selected strains to fludioxonil ranged from 2.810 x 10-9 to 0.740 9 mg/L,which were extremely sensitive to fludioxonil.No resistant popu-lation was found,and the mean EC50 value was(0.024 9±0.093 6)mg/L,indicating that rice F.fujiku-roi in Jilin province did not develop resistance to fludioxonil.Compared with their parent strains,re-sistance levels of the two resistant mutants(LH10-2-11 and DH34-5-11)were 3 596.48 and 9 539.73 fold,respectively,showing high resistance.Through biological testing,adaptability of resistant mu-tants was proved to be extremely high,making them easy to become dominant fungi in the field.The results of cross resistance test showed that there was positive cross resistance between fludioxonil and thiram,thiophanate-methyl,metalaxyl-M and hymexazd,but no cross resistance with prochloraz and tebuconazole.The results suggested that the resistant mutants of F.fujikuroi from rice can be de-veloped under the selection of fludioxonil,which poses a risk of resistance to fludioxonil.The results lay foundation for the control of rice bakanae caused by F.fujikuroi and management of fungicide re-sistance of F.fujikuroi to fludioxonil.关键词
水稻恶苗病菌/咯菌腈/敏感性/抗药突变体/生物学性状/抗药性治理/吉林省Key words
Fusarium fujikuroi/fludioxonil/sensitiveness/resistant mutant/biological character-istic/resistance management/Jilin province分类
农业科技引用本文复制引用
刘禹含,邹佳营,何博,刘丽萍,高洁..吉林省水稻恶苗病菌对咯菌腈的敏感性及抗药性风险评估[J].吉林农业大学学报,2024,46(2):211-218,8.基金项目
国家重点研发计划项目(2018YFD0300200),国家级大学生创新创业训练计划项目(201810193042) （2018YFD0300200）
