吉林农业大学学报2024，Vol.46Issue(2)：219-227,9.DOI:10.13327/j.jjlau.2021.1560
2个抗感烟草品种响应马铃薯Y病毒早期胁迫的转录组分析
Transcriptome Analysis of Two Resistant and Susceptible Tobacco Varieties in Response to Early Potato Y Virus Stress
摘要
Abstract
In order to explore the response differences of different tobacco resistant varieties NC95 and SY04-3 to potato virus Y stress,tobacco seedlings were inoculated with PVY,and seedling samples were collected at 12 h.Transcriptome sequencing and bioinformatics analysis were per-formed.The analysis results of PVY inoculation and control showed that NC95 up-regulated 206 dif-ferentially expressed genes and down-regulated 111 genes.SY04-3 up-regulated 1 001 differentially expressed genes,down-regulated 423.The functions of these differentially expressed genes are classi-fied into 54 GO entries in three categories:biological processes,cell components and molecular func-tions.NC95 and SY04-3 up-regulated differentially expressed genes were significantly enriched in 7 KEGG metabolic pathways,while NC95 and SY04-3 down-regulated differentially expressed genes were significantly enriched in 10 and 14 KEGG metabolic pathways,respectively;Transcription fac-tors predicted that 20 transcription factors in 13 families of NC95 changed significantly,and 38 tran-scription factors in 20 families of SY04-3 changed significantly.This study provides reference for the study on the early response of tobacco to potato Y virus infection mechanism.关键词
烟草/马铃薯Y病毒/转录组/差异表达基因Key words
tobacco/potato virus Y/transcriptome/differentially expressed genes分类
农业科技引用本文复制引用
王平,任雅萍,任昭辉,苏亮,吴委林,杨超群,董环宇,朴世领..2个抗感烟草品种响应马铃薯Y病毒早期胁迫的转录组分析[J].吉林农业大学学报,2024,46(2):219-227,9.基金项目
国家自然科学基金项目(31760418) （31760418）
