电针预处理通过调控AKT/mTOR信号通路减轻缺血再灌注大鼠心肌损伤OACSTPCD
Electro-acupuncture pretreatment alleviating myocardial injury in ischemia-reperfusion rats by regulating AKT/mTOR pathway
目的 观察电针预处理对心肌缺血再灌注损伤大鼠的影响并探讨其作用机制.方法 30只SD大鼠随机抽取6只为假手术组(只穿线不结扎),剩余大鼠采用冠脉结扎法建立心肌缺血再灌注损失(MIRI)大鼠模型,并在造模前1周分别进行电针内关预处理、雷帕霉素预处理和雷帕霉素+电针内关预处理.造模结束后用HE染色法检测心肌细胞病理形态学变化;ELISA法检测血清中CK-MB和IL-6含量;Western-blot法检测Beclin1、Caspase3及磷酸化的AKT、mTOR蛋白表达.结果 与模型组比较,电针组心肌纤维断裂减少、细胞间质出血减少,其损伤程度与假手术组较为接近;与雷帕霉素组比较,雷帕霉素+电针组心肌纤维断裂减少、细胞水肿程度减轻.与假手术组比较,模型组大鼠CK-MB、IL-6、Beclin1和Caspase3表达明显升高,p-AKT、p-mTOR表达明显下调(P＜0.01);与模型组比较,电针组大鼠CK-MB、IL-6、Beclin1和Caspase3表达明显下调,p-AKT、p-mTOR表达明显升高(P＜0.01);与电针组比较,雷帕霉素+电针组大鼠IL-6、CK-MB、Beclin1和Caspase3表达明显升高(P＜0.05或P＜0.01),p-AKT、p-mTOR表达明显下调(P＜0.01).结论 电针内关预处理减轻大鼠MIRI可能与激活AKT/mTOR信号通路相关.
Objective To observe the effect of electro-acupuncture pretreatment on myocardial ischemia-reperfusion injury(MIRI)in model rats and explore its mechanism.Methods Six SD rats were randomly selected from the 30 SD rats for a sham operation group(only threading without ligature),while an MIRI rat model was established by coronary ligation in the remaining rats,and the electro-acupuncture pretreatment,or rapamycin pretreatment,or rapamycin+electro-acupuncture pretreatment were performed on the remaining rats one week before modeling.HE staining was used to detect the pathological changes of cardiomyocytes after the modeling.The contents of CK-MB and IL-6 in serum were detected by ELISA.The protein expressions of Beclin1,Caspase3 and phosphorylated AKT and mTOR were detected by Western blot.Results Compared with the model group,the myocardial fiber breakage and interstitial bleeding were decreased in the electro-acupuncture group,and its damage degree was similar to that in the sham operation group.Compared with the rapamycin group,the myocardial fiber breakage and the degree of cell edema were reduced in the rapamycin+electro-acupuncture group.Compared with the sham operation group,the expressions of CK-MB,IL-6,Beclin1 and Caspase3 in the model group were significantly increased,while the expressions of p-AKT and p-mTOR were significantly down-regulated(P<0.01).Compared with the model group,the expressions of CK-MB,IL-6,Beclin1 and Caspase3 in the electro-acupuncture group were significantly down-regulated,while the expressions of p-AKT and p-mTOR were significantly increased(P<0.01).Compared with the electro-acupuncture group,the expressions of IL-6,CK-MB,Beclin1 and Caspase3 in the rapamycin+electro-acupuncture group were significantly increased(P<0.05 or P<0.01),while the expressions of p-AKT and p-mTOR were significantly down-regulated(P<0.01).Conclusion The electro-acupuncture pretreatment at Neiguan point can alleviate MIRI in rats,which is supposed to be related to the activation of the AKT/mTOR signaling pathway.
贺春艳;李丹;张燚格;何妙嬴;柳冬冬;唐雅妮;王超
湖南中医药大学针灸推拿与康复学院,长沙 410208长沙民政职业技术学院,长沙 410004
中医学
电针预处理内关细胞自噬心肌缺血再灌注损伤AKT/mTOR信号通路
electro-acupuncture pretreatmentNeiguan(P6)autophagymyocardial ischemia-reperfusion injuryAKT/mTOR signaling pathway
《吉林中医药》 2024 (005)
559-563 / 5
湖南省自然科学基金资助项目(2021JJ30507);湖南省教育厅优秀青年项目(23B1113)
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