南京农业大学学报2024，Vol.47Issue(3)：453-461,9.DOI:10.7685/jnau.202305013
绿豆GPX基因家族鉴定及其对非生物胁迫的响应
Identification of the GPX gene family in Vigna radiata and its response to abiotic stress
摘要
Abstract
[Objectives]This research used bioinformatics methods to screen and identify the GPX gene family in Vigna radiata(mung bean),and to explore its expression pattern and function in abiotic stress.[Methods]The isoelectric point,molecular weight,collinearity and cis-acting elements of the GPX gene family in mung bean were analyzed by bioinformatics methods.The expression patterns of the GPX gene family in mung bean and its expression levels under different abiotic stresses,and hormones were further analyzed by transcriptome data.Transcriptome sequencing and RT-qPCR were used to further analyze the tissue expression patterns and the expression levels of GPX gene family in V.radiata under different stress and hormones.[Results]Total of 8 GPX genes were identified in mung bean genome,which were unevenly distributed on chromosome 1/2/3/5/10.According to the gene structure and motif analysis,the eight GPX genes had similar structures,all containing six intronic regions,and most of the GPX members had UTR regions.Transcriptome and RT-qPCR analysis showed that multiple GPX members were able to respond to a variety of stress.Based on the expression of GPX in different tissues,different GPX members were specifically expressed in different tissues.GPX expression in mung bean was low and might be strongly induced under stress.[Conclusions]The GPX gene family of mung bean responded to a variety of abiotic stresses,and different GPX members responded to different types of stress.关键词
绿豆/GPX基因家族/非生物胁迫/基因表达Key words
mung bean/GPX gene family/abiotic stress/gene expression分类
农业科技引用本文复制引用
邱涛涛,苏鸿飞,王瑜,张禾佳,胡慧欣,阮诗婷,袁星星,谢彦杰..绿豆GPX基因家族鉴定及其对非生物胁迫的响应[J].南京农业大学学报,2024,47(3):453-461,9.基金项目
江苏省基础研究计划杰出青年基金项目(BK20220084) （BK20220084）
江苏省基础研究计划青年基金项目(BK20200282) （BK20200282）
