基于生物信息学分析干性相关基因TCEAL7作为胃癌预后标志物的研究OA北大核心CSTPCD
Bioinformatics analysis of stemness-related gene TCEAL7 as a prognostic factor in gastric cancer
目的:在胃癌(gastric cancer,GC)中寻找与基于mRNA表达水平的干性指数(mRNA expression-based stemness in-dex,mRNAsi)相关的基因,并探讨其功能.方法:通过癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库和GSE66229数据集进行生物信息学分析.通过实时定量聚合酶链反应(qRT-PCR)和免疫组织化学评估组织和细胞系中TCEAL7的表达.采用过表达TCEAL7的GC细胞研究TCEAL7对GC细胞增殖、迁移和侵袭能力以及干性特征的影响.结果:TCEAL7在GC中表达下调.但TCEAL7的高表达与较差的预后相对应.过表达TCEAL7抑制GC细胞的增殖、迁移和侵袭能力,减弱成球能力并减少干细胞样CD44high/CD24high细胞的数量.包含临床特征和TCEAL7表达的列线图对于预测GC患者预后有很好的准确性和区分度.结论:TCEAL7被鉴定为在GC中与mRNAsi相关的预后因子,它降低了肿瘤细胞的恶性程度,并减弱了细胞的干性特征.
Objective:To identify genes associated with mRNA expression-based stemness index(mRNAsi)in gastric cancer(GC)and explore their functions.Methods:Bioinformatics analysis was conducted using The Cancer Genome Atlas(TCGA)database and microarray GSE66229 dataset.The expression of TCEAL7 in tissues and cell lines was assessed by quantitative real-time polymerase chain reaction(qRT-PCR)and immunohistochemistry.The effect of TCEAL7 overexpression on the proliferation,migration,invasion,and stemness characteristics of GC cells was studied.Results:The expression of TCEAL7 was downregulated in GC.High expression of TCEAL7 was associated with poorer prognosis.Overexpression of TCEAL7 inhibited the proliferation,migration and invasion of GC cells,attenuated spheroid formation,and decreased the population of stem-like CD44high/CD24high cells.The nomogram which included clinical features and TCEAL7 expression showed good accuracy and discrimination for predicting the prognosis of patients with GC.Conclusions:TCEAL7 was identified as a mRNAsi-related prognostic factor in GC,inhibiting the malignancy of tumor cells and attenuating stemness characteristics.
刘嘉浩;钱海声;高欣;李文洁;马蕊;杨振;李璇;张国新
南京医科大学第一附属医院消化内科,江苏 南京 210029江苏卫生职业健康学院护理学院,江苏 南京 211800泰州市人民医院消化内科,江苏 泰州 225300
临床医学
胃癌mRNA表达水平的干性指数预后
gastric cancermRNA expression-based stemness indexprognostic
《南京医科大学学报（自然科学版）》 2024 (006)
769-780 / 12
国家自然科学基金(81970499)
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