小麦TaPR1基因启动子分析及其调控因子的筛选OA北大核心CSTPCD

Pathogenesis-related protein 1(PR1)is an important defense protein in plants,its production and accumulation in plants is one of the important responses to biotic and abiotic stresses.In previous study,TaPR1 was found to be induced by Puccinia triticina(Pt)and participate in wheat defense response to leaf rust.In the early stage of this study,the TaPR1 promoter sequence was successfully obtained by gene cloning and named pTaPR1,among which the 900 bp sequence(pTaPR1900)had strong activity.This study further analyzed the sequence composition and possible regulatory factors of pTaPR1.Preliminary analysis based on the PLACE and PlantCARE databases showed that pTaPR1 contained the core elements including TATA-box,CAAT-box and a large number of light-responsive elements,as well as the ABA-responsive element and the MYB-binding site.Yeast one-hybrid(Y1H)technique was used to screen the transcription factors that might bind to pTaPR1900 involved in regulating TaPR1 gene expression,12 candidates were obtained.The interaction between 4 candidates including U-box E3 ubiquitin ligase,pollen protein,glycine A3 protein and heavy metal-associated isoprenoid phycobiliprotein with pTaPR1900 were initially verified.Taken together,this study provides theoretical reference for exploring the transcriptional regulatory mechanism of TaPR1.