农业生物技术学报2024，Vol.32Issue(6)：1297-1306,10.DOI:10.3969/j.issn.1674-7968.2024.06.006
东方百合'西伯利亚'LoNAC48基因的克隆、表达及功能分析
Cloning,Expression and Functional Analysis of LoNAC48 Gene in Lilium Oriental Hybrid 'Siberia'
摘要
Abstract
The long cultivation cycle and poor quality of lily bulbs by produced in China is a important factors that lead to long-term dependence on imports.The plant-specific transcription factor NAC plays an important role in regulating plant growth and development,resistance establishment,etc.,and mining,identifying and studying related genes in lily is a strategy to solve the above problems.In this study,one gene transcript carrying NAC domain was screened in the lily transcriptome database,and the gene was cloned and identified as LoNAC48 gene(GenBank No.PP078617)based on PCR.Bioinformatics analysis showed that LoNAC48 gene contained a complete open reading frame with a length of 777 bp encoding 258 amino acids,which was an unstable hydrophobic protein,and phylogenetic tree analysis showed that it was highly homologous to Lilium regale LrNAC48 protein.The results of subcellular localization showed that LoNAC48 was a nuclear transcription factor,and tissue expression analysis showed that LoNAC48 gene was present in bulbs,roots,flowers and other tissues and organs expression,among which the expression level was the highest in leaves;The results of different stress responses showed that the expression of LoNAC48 gene was susceptible to low temperature,high temperature,drought,salt and hydrogen peroxide(H2O2)stresses,Under low temperature,salt,and hydrogen peroxide stress,the expression level was significantly upregulated at 12 hours compared to the control,and significantly decreased at 24 hours of drought stress,and the expression pattern of LoNAC48 was almost the same under salt stress and hydrogen peroxide stress.Phenotypic analysis of transgenic Arabidopsis thaliana lines showed that overexpression of LoNAC48 gene could significantly promote the growth of Arabidopsis thaliana and increase the leaf area.These results indicated that LoNAC48 gene had the dual functions of stress resistance and regulation of plant growth and development.This study provides genetic resources for molecular-assisted breeding of lily and improvement of lily traits.关键词
百合/LoNAC48/基因克隆/生物信息学分析/功能分析Key words
Lily/LoNAC48/Gene cloning/Bioinformatics analysis/Functional analysis分类
农业科技引用本文复制引用
胡晓聪,王可,孙亮..东方百合'西伯利亚'LoNAC48基因的克隆、表达及功能分析[J].农业生物技术学报,2024,32(6):1297-1306,10.基金项目
2023年度浙江省高校国内访问工程师"校企合作项目"(FG2023011) （FG2023011）
