二穗短柄草RING Finger基因家族的鉴定及表达分析OA北大核心CSTPCD

RING finger proteins,which usually have E3 ubiquitin ligase activity,are important in regulating plant growth and development and in response to biotic and abiotic stresses.However,studies on the RING finger gene family of Brachypodium distachyon are limited.In this study,the RING finger gene family of B.distachyon was systematically analyzed using bioinformatics methods,and its expression under drought,salt,and abscisic acid(ABA)stress treatments was preliminarily analyzed by qPCR.The results showed that a total of 666 RING finger genes were identified in the B.distachyon genome,which were unevenly distributed on 5 chromosomes,and 39 intergenic duplications occurred.The phylogenetic analysis divided the RING finger gene family into 9 subclusters,and the members of each subcluster were highly homologous and contained similar conserved structural domains and motifs.The covariance analysis among species showed that the RING finger gene family originated from genome duplication,and that gene fragment duplications and tandem duplications played a key role in its expansion and evolution.There were 303 RING finger collinearity gene pairs in rice(Oryza sativa)and B.distachyon,whereas there were only 48 pairs in Arabidopsis thaliana and B.distachyon,and a total of 39 RING finger gene pairs in B.distachyon genomes.Tissue expression profiles showed that 547 RING finger genes were expressed in different tissues of B.distachyon,and 39.8%of the genes were highly expressed in male flowers of B.distachyon.Differential expression analysis showed that 29 RING finger genes might be involved in the response to drought,salt and abscisic acid(ABA)stress.qPCR results showed that 26 RING finger genes were significantly up-regulated in drought stress(P＜0.05),3 RING finger genes were down-regulated,27 RING finger genes were up-regulated and 2 were down-regulated in salt stress;15 RING finger genes were up-regulated,9 RING finger genes were down-regulated,and 5 RING finger genes were at the same level as the control in ABA stress.The present study provides a theoretical basis for revealing the function and evolution of the RING finger gene family and its role in the regulation of drought stress.