Hippo信号通路下游关键因子YAP和TEAD-1在牦牛胎盘发育中的表达与定位OA北大核心CSTPCD

Yes-associated protein(YAP)and TEA domain transcription factor 1(TEAD-1)are the core factors of the Hippo signaling pathway and play an important role in regulating cell proliferation and organ growth.In order to detect the expression of YAP and TEAD-1 in different developmental stages of yak(Bos grunniens)placenta,and to explore the physiological role of Hippo signaling pathway in regulating placental cell proliferation and organ growth regulation.In this study,placental tissue samples of pregnant yaks were collected and divided into different developmental stages(＜2 months old,3 months old,4 months old,＞5 months old)based on fetal head-to-hip distance.The expression of YAP and TEAD-1 was detected and analyzed by qRT-PCR,Western blot(WB)and Immunohistochemistry(IHC)technology were used to detect the expression of these genes.The results showed that YAP and TEAD-1 were mainly expressed in fetal chorionic trophoblast giant cells,columnar trophoblast cells and maternal placental crypt epithelial cells.With the progress of pregnancy,the expression of YAP and TEAD-1 mRNA and protein in yak placenta tissue change significantly,and the change trend of YAP and TEAD-1 was basically the same.The co-expression characteristics and the correlation between the expression level and the placental development stage suggested that YAP and TEAD-1 might be involved in the regulation of yak placental development through the Hippo signaling pathway,and play an important role in the physiological function of placental tissue and pregnancy maintenance.The results provides a theoretical basis for elucidating the mechanism of yak placental development and pregnancy regulation.