药学研究2024，Vol.43Issue(5)：428-434,7.DOI:10.13506/j.cnki.jpr.2024.05.003
阿魏酸治疗阿尔茨海默病的潜在靶点预测及验证
Prediction and validation of potential targets for Alzheimer's disease treatment using ferulic acid
摘要
Abstract
Objective To predict the potential targets of ferulic acid in the treatment of Alzheimer's disease(AD)using bioinformatics and molecular biology,and investigat its efficacy in AD.Methods The methodology involved obtaining genes associated with the interaction between AD and ferulic acid from the DisGeNET and PubChem databases,conducting GO/KEGG enrichment analysis of common targets using the DAVID online database,and establishing a protein-protein interaction network to identify key genes through the STRING database;And analyzing the expression of key genes through Western blotting and ELISA.Results After identifying 38 common interaction targets,the construction of a protein-protein interaction network revealed the top three genes,namely MMP9,TLR4,and ACE.In Western blotting and ELISA,ferulic acid was found to downregulate the expression of MMP9,TLR4,and ACE(P<0.05).Conclusion The results suggest that MMP9,TLR4,and ACE may serve as potential therapeutic targets for ferulic acid in the treatment of AD.关键词
阿尔茨海默病/阿魏酸/生物信息学/基质金属蛋白酶9/Toll样受体4/血管紧张素转化酶Key words
Alzheimer's disease/Ferulic acid/Bioinformatics/MMP9/TLR4/ACE分类
中医学引用本文复制引用
陈斯亮,刘梦鸽,史晋宁,徐晓庆,蔡红艳,韩维娜..阿魏酸治疗阿尔茨海默病的潜在靶点预测及验证[J].药学研究,2024,43(5):428-434,7.基金项目
国家自然科学基金面上项目(No.82171428) （No.82171428）
湖南省自然科学基金青年项目(No.2020JJ5517) （No.2020JJ5517）
湖南省教育厅科学研究项目(No.20C1671) （No.20C1671）
国家级大学生创新创业训练项目(No.S202010547043) （No.S202010547043）
