山东农业科学2024，Vol.56Issue(5)：138-144,7.DOI:10.14083/j.issn.1001-4942.2024.05.018
棉花黄萎病生防菌筛选鉴定及根瘤菌DG3-1对棉花生长的影响
Screening and Identification of Biocontrol Bacteria for Cotton Verticillium Wilt and Effect of Rhizobium DG3-1 on Cotton Growth
摘要
Abstract
The aim of this study was to explore the germplasm resources of endophytic bacteria in south-ern Xinjiang cotton for the prevention and control of cotton Verticillium wilt,and also explore the impact of rhi-zobia on cotton growth.Endophytic bacteria were isolated from different tissues of cotton using conventional tis-sue separation method.Antagonistic endophytic bacteria against Verticillium dahliae were screened by plate confrontation method,and then molecular identification was performed.The effects of rhizobia on cotton growth were studied by pot experiments.The results showed that there were certain differences in the number of endo-phytic bacteria in different tissues of cotton and that in roots was the most,followed by leaves and stems.A-mong the 31 strains of isolated endophytic bacteria,11 showed antagonistic activity against cotton V.dahliae with inhibition rates of 35.99%～68.28%.After identification,9 strains of Bacillus and 2 strains of Rhizobium were identified,among which Rhizobium DG3-1 had a inhibition rate of(56.63±1.82)%against cotton V.dahliae.The results of the pot experiment showed that compared to the control,the aboveground fresh weight,aboveground dry weight,underground fresh weight,underground dry weight and chlorophyll content in cotton plants treated with strain DG3-1 increased by 2.12,2.09,1.05,0.75 and 1.75 times,respectively.Strain DG3-1 had good antibacterial effect against cotton V.dahliae and certain promoting effect on cotton growth,which was a potential strain for cotton V.dahliae prevention and control,and could provide strain resources for the development of microbial agents.关键词
棉花/内生细菌/棉花黄萎病/拮抗/根瘤菌DG3-1/促生Key words
Cotton/Endophytic bacteria/Cotton Verticillium wilt/Antagonism/Rhizobium DG31/Growth promotion分类
农业科技引用本文复制引用
代先兴,闫成才,祖丽胡玛尔·麦提喀迪尔,王庆朋,冯俊辉,李猛,王兰,牛冬冬,郝海婷..棉花黄萎病生防菌筛选鉴定及根瘤菌DG3-1对棉花生长的影响[J].山东农业科学,2024,56(5):138-144,7.基金项目
塔里木大学校长基金资助项目(TDZKJC201801) （TDZKJC201801）
南京农业大学-塔里木大学科研联合基金资助项目(NNLH202002) （NNLH202002）
塔里木大学研究生科研创新项目(TDGRI202224) （TDGRI202224）
塔里木大学大学生科研创新项目(2023014) （2023014）
