基于微卫星标记的江西桃红岭梅花鹿遗传多样性OA北大核心CSTPCD

Sika deer(Cervus nippon)is a Class I key protected wild animal in China.The Jiangxi Taohongling sika deer represents the largest population of South China sika deer(C.n.kopschi).Studying the genetic diversity of Tao-hongling sika deer and understanding its genetic background is crucial for enhancing the genetic diversity of sika deer in South China and promoting the population's rapid development.This study collected a total of 108 suspected sika deer fecal samples from the Taohongling Sika Deer National Nature Reserve.Mitochondrial Cyt b gene amplification se-quencing was used for species identification,while microsatellite markers and SRY/ZFX sex markers were employed for individual identification,genetic diversity analysis,and sex determination.The results showed that 96 out of the 108 samples were produced by 63 individual sika deer,resulting in a sex ratio of 1.33 males to 1 female.The average num-ber of alleles,observed heterozygosity,expected heterozygosity,and inbreeding coefficient of the 7 microsatellite loci were 3.714,0.712,0.602,and-0.087,respectively.Three loci significantly deviated from Hardy-Weinberg equilibri-um and all showed heterozygote excess.These findings suggest that the genetic diversity of Taohongling sika deer is moderate,with a certain degree of sexual imbalance.Furthermore,a negative inbreeding coefficient indicates that there is no current risk of inbreeding in the population.To improve the conservation and management of rare alleles,it is ad-visable to implement artificial breeding programs for the Taohongling sika deer.Additionally,measures such as promot-ing individual migration should be implemented to facilitate genetic exchange between the Taohongling sika deer and other South China sika deer populations.This will help maintain their population genetic diversity and promote rapid de-velopment.