兽类学报2024，Vol.44Issue(3)：268-276,9.DOI:10.16829/j.slxb.150803
基于微卫星标记的江西桃红岭梅花鹿遗传多样性
Genetic diversity of sika deer based on microsatellite in Taohongling,Jiangxi
摘要
Abstract
Sika deer(Cervus nippon)is a Class I key protected wild animal in China.The Jiangxi Taohongling sika deer represents the largest population of South China sika deer(C.n.kopschi).Studying the genetic diversity of Tao-hongling sika deer and understanding its genetic background is crucial for enhancing the genetic diversity of sika deer in South China and promoting the population's rapid development.This study collected a total of 108 suspected sika deer fecal samples from the Taohongling Sika Deer National Nature Reserve.Mitochondrial Cyt b gene amplification se-quencing was used for species identification,while microsatellite markers and SRY/ZFX sex markers were employed for individual identification,genetic diversity analysis,and sex determination.The results showed that 96 out of the 108 samples were produced by 63 individual sika deer,resulting in a sex ratio of 1.33 males to 1 female.The average num-ber of alleles,observed heterozygosity,expected heterozygosity,and inbreeding coefficient of the 7 microsatellite loci were 3.714,0.712,0.602,and-0.087,respectively.Three loci significantly deviated from Hardy-Weinberg equilibri-um and all showed heterozygote excess.These findings suggest that the genetic diversity of Taohongling sika deer is moderate,with a certain degree of sexual imbalance.Furthermore,a negative inbreeding coefficient indicates that there is no current risk of inbreeding in the population.To improve the conservation and management of rare alleles,it is ad-visable to implement artificial breeding programs for the Taohongling sika deer.Additionally,measures such as promot-ing individual migration should be implemented to facilitate genetic exchange between the Taohongling sika deer and other South China sika deer populations.This will help maintain their population genetic diversity and promote rapid de-velopment.关键词
梅花鹿/微卫星/个体识别/遗传多样性/性别比例Key words
Sika deer/Microsatellite/Individual identification/Genetic diversity/Sex ratio分类
生物科学引用本文复制引用
张阳,陈璐瑶,韩卫杰,詹建文,刘武华,黄晓凤..基于微卫星标记的江西桃红岭梅花鹿遗传多样性[J].兽类学报,2024,44(3):268-276,9.基金项目
江西省重点研发计划项目(20212BBG73016) （20212BBG73016）
江西省林业局林业科技创新专项(创新专项[2021]05号) （创新专项[2021]05号）
江西省林业科学院基础研究与人才科研专项(2023521603,2023521606) （2023521603,2023521606）
