山栀子不同部位活性成分含量及抗氧化活性OA北大核心CSTPCD

To compare the active compounds in different parts of Gardenia jasminoides Ellis and evaluate their in vitro antioxidant activities,this study used the ultrasound-assisted method to extract the active compounds in different parts of Gardenia jasminoides Ellis and measured the yield.Their total iridoids,total crocins,total flavonoids and total polyphenols were measured by UV-visible spectrophotometer method.Their active compound monomers were measured by HPLC method.DPPH and ABTS+radical scavenging methods were used to valuate their in vitro antioxidative activities then the correlation analysis was founded between the contents of active compounds and IC50 of DPPH and ABTS+radical.Gardenia jasminoides Ellis leaf had the highest total iridoids contents and total polyphenols contents,which were 244.87±5.89 mg·g-1and 16.71±0.55 mg·g-1 respectively.Results showed that,theGardenia jasminoides Ellis fruit had the significantly highest total crocins contents(P＜0.05),which was 16.18±1.40 mg·g-1,Crocin-Ⅰ was the main form of crocin,which occupied about 60％of the total crocin contents.Gardenia jasminoides Ellis flower had the highest total flavonoids contents and,which was 43.61±1.01 mg·g-1.According to DPPH and ABTS+radical scavenging experiments,the Gardenia jasminoides Ellis leaf had the best antioxidative activities,which IC50 of DPPH and ABTS+were 36.00±2.70 and 31.96±3.87 μg·mL-1.The correlation analysis showed that iridoids compounds and polyphenols compounds had strong correlation with antioxidative activities.Among the different parts of Gardenia jasminoides Ellis extracted by ultrasound-assisted method,it was found that different parts had certain antioxidant capacity.Gardenia jasminoides Ellis leaf had antioxidative activities beyond the fruit,which provided theoretical sources for development of natural antioxidants.