食品工业科技2024，Vol.45Issue(12)：205-214,10.DOI:10.13386/j.issn1002-0306.2023090277
金花茶多酚制备、成分分析及其体内外抗氧化活性分析
Preparation and Component Analysis of Camellia nitidissima Chi Polyphenols and Their Antioxidant Activites in Vivo and in Vitro
摘要
Abstract
This study aimed to investigate the optimal extraction process for polyphenols from Camellia nitidissima Chi and to analyze the total polyphenol composition and antioxidant activities both in vivo and in vitro post-purification.Camellia nitidissima Chi was the raw material and a response surface methodology based on single factor experiments was designed to screen the optimal extraction parameters including ultrasound power,extraction temperature,ethanol concentration and solid-liquid ratio.The extract was purified using Lx-8 macroporous resin,and five specific polyphenolic monomers were qualitatively and quantitatively analyzed using high-performance liquid chromatography(HPLC).Subsequently,in vivo and in vitro antioxidant experiments were conducted on the extract.The results showed that the optimal conditions were an ultrasonic power of 290 W,an extraction temperature of 35 ℃,an ethanol concentration of 55％,and a material-liquid ratio of 1∶30 g/mL.Under these conditions,the yield of polyphenols from Camellia nitidissima Chi was 3.53％,the purity was 63.73％.The content of five polyphenolic monomer compounds,namely gallic acid,chlorogenic acid,epicatechin,rutin,and ellagic acid was 0.47,11.18,59.03,18.34,and 16.41 mg/g,respectively.In addition,the polyphenols extracted from Camellia nitidissima Chi demonstrated a significant ability to boost oxidative damage resistance in C.elegans.This was evidenced by a marked increase in SOD(superoxide dismutase)enzyme activity and a decrease in MDA(malondialdehyde)levels within the C.elegans specimens.关键词
金花茶多酚/超声辅助提取/响应面法/成分分析/秀丽隐杆线虫/抗氧化损伤Key words
Camellia nitidissima Chi polyphenols/ultrasound-assisted extraction/response surface methodology/compositional analysis/Caenorhabditis elegans/antioxidant damage分类
轻工纺织引用本文复制引用
许嘉文,孙庆,阿丽娅·阿布杜热依木,唐伟,阮琳,孙秀岩,解军波..金花茶多酚制备、成分分析及其体内外抗氧化活性分析[J].食品工业科技,2024,45(12):205-214,10.基金项目
天津市"131"创新型人才项目(201927). （201927）
