食品工业科技2024，Vol.45Issue(12)：319-326,8.DOI:10.13386/j.issn1002-0306.2023070031
不同灵芝菌丝体水提物对乙醇诱导LO2肝细胞损伤的保护作用
Protective Effects of Water Extracts from Different Ganoderma lucidum Mycelium on Ethanol-induced Hepatocyte Injury in LO2 Cells
摘要
Abstract
To explore the protective effects of water extracts from different Ganoderma lucidum(G.lucidum)mycelium on ethanol-induced hepatocyte injury in LO2 cells.The effects of water extracts obtained from different G.lucidum mycelium against ethanol-induced damage to LO2 cells were examined in this research.Cell survival rate,alanine aminotransferase(ALT),aspartate aminotransferase(AST),reactive oxygen species(ROS),superoxide dismutase(SOD),glutathione(GSH)and malondialdehyde(MDA)levels were measured in a liver injury model employing ethanol-induced LO2 cells,to assess the preventive effects of G.lucidum mycelium water extracts against ethanol-induced damage.The results showed that water extracts from the mycelium of all seven strains exhibited varying degrees of protective effects.Among them,the water extract from strains G0119 and G0154 had the best protective effect.Compared with the model group,the intervention of water extracts significantly increased LO2 cell survival(P＜0.05),while decreased ALT and AST levels in the cell culture medium,respectively(P＜0.05).Furthermore,the intracellular ROS level significantly decreased(P＜0.05),while the activity of SOD and the content of GSH significantly increased(P＜0.05),and the MDA content significantly decreased compared to the model group(P＜0.05).Correlation analysis revealed a positive correlation between the molecular weight of polysaccharide component 2 in the water extract and its hepatoprotective activity,with higher molecular weight correlating with stronger activity.The results indicated that the water extract of G.lucidum mycelium exhibited a protective effect against ethanol-inducedinvitro liver damage.Moreover,the effectiveness of the G0119 and G0154 strains were superior,and its hepatoprotective effect was correlated with the molecular weight of polysaccharide components in the extract.关键词
灵芝/菌丝体/水提物/乙醇性肝损伤/保肝作用Key words
Ganoderma lucidum/mycelium/water extract/ethanol-induced liver injury/hepatoprotective effects分类
轻工纺织引用本文复制引用
陈亚平,刘利平,冯杰,刘艳芳,唐传红,颜梦秋,张劲松,韩伟..不同灵芝菌丝体水提物对乙醇诱导LO2肝细胞损伤的保护作用[J].食品工业科技,2024,45(12):319-326,8.基金项目
上海市现代农业产业技术体系(沪农科产字(2023)第9号) （沪农科产字(2023）
上海市农业科学院卓越团队建设计划资助(沪农科卓(2022)003). （沪农科卓(2022）
