食品工业科技2024，Vol.45Issue(12)：351-357,7.DOI:10.13386/j.issn1002-0306.2023090242
木糖对淀粉体外消化性的影响及作用机理
Effect of Xylose on in Vitro Digestion of Starch and Its Action Mechanism
摘要
Abstract
Starch was the main source of carbohydrates in people's daily diet,and its digestibility was an important factor affecting the body's postprandial blood glucose level.In this paper,the effect of xylose on the in vitro digestive properties of corn starch and its mechanism of action were investigated.The in vitro digestibility of starch was evaluated by an in vitro simulated digestion system after different additions of xylose(5％,10％,and 15％),and the mechanisms of xylose on α-amylase and α-glucosidase,as well as its effect on blood glucose fluctuation through enzyme inhibition kinetics,molecular docking,and human glycemic index(GI)tests.The results showed that the digestive hydrolysis of corn starch was inhibited by different amounts of xylose,and the IC50 values of xylose on α-glucosidase and α-amylase were 205.35 and 4.75 mg/mL,respectively,which belonged to the mixed type inhibitors.Xylose interacted with amino acid residues of α-glucosidase andα-amylase through hydrogen bonding with binding energies of-3.05 and-5.30 kcal/mol,respectively.Additionally,the GI value of plant-based peptidoglycan milk decreased from 58 to 45 after the addition of xylose,which demonstrated that xylose was effective in decreasing the fluctuation of postprandial blood glucose.The results could provide scientific data to support the research and application of xylose in nutritional and health foods.关键词
木糖/淀粉消化性/α-淀粉酶/α-葡萄糖苷酶/血糖波动Key words
xylose/starch digestibility/α-amylase/α-glucosidase/glucose fluctuations分类
轻工纺织引用本文复制引用
王萌,陈孟泽,柳嘉,刘士伟,马喜山,苑鹏,晏永球,王俊,段盛林..木糖对淀粉体外消化性的影响及作用机理[J].食品工业科技,2024,45(12):351-357,7.基金项目
宁波市公益类科技计划项目(植物基肽乳制品创制关键技术与产业化应用)(2021S001). （植物基肽乳制品创制关键技术与产业化应用）
