食品科学2024，Vol.45Issue(10)：45-53,9.DOI:10.7506/spkx1002-6630-20230906-051
辣椒素与槲皮苷通过EGFR/PI3K/Akt信号通路调节HepG2细胞脂质代谢作用机制
Mechanism of Action of Capsaicin and Quercitrin in Regulating Lipid Metabolism in HepG2 Cells through EGFR/PI3K/Akt Signaling Pathway
摘要
Abstract
The objective of this study was to investigate the molecular mechanism behind the regulatory effect of capsaicin combined with quercitrin on liver lipid metabolism.The effects of capsaicin alone or in combination with quercitrin on the survival rate of HepG2 cells with oleic acid-induced lipid accumulation were investigated.Oil red O staining was used to observe lipid accumulation in HepG2 cells.Meanwhile,the levels of total triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)and total bile acid(TBA)were determined.Moreover,the expression levels of epidermal growth factor receptor(EGFR),phosphatidylinositol 3-kinase(PI3K),protein kinase B(Akt),farnesoid X receptor 1(FXR1),cholesterol 7α-hydroxylase(CYP7A1)and fibroblast growth factor 19(FGF19)were determined by Western blotting.The results showed that the proliferation rate of HepG2 cells in each treatment group was greater than 75％,demonstrating no significant cytotoxicity.The results of oil red O staining showed a reduction in lipid accumulation in both single and combined treatment groups.The application of capsaicin alone or combined with quercitrin reduced the contents of TG,TC and LDL-C,increased the contents of HDL-C and TBA,and up-regulated the protein expression levels of EGFR,PI3K,Akt,FXR1 and FGF19.In summary,capsaicin combined with quercitrin exerted a synergistic regulatory effect on lipid metabolism in HepG2 cells,with the most pronounced effect being observed at a 3:1 ratio.关键词
辣椒素/槲皮苷/HepG2细胞/脂质代谢/分子机制Key words
capsaicin/quercitrin/HepG2 cells/lipid metabolism/molecular mechanism分类
轻工业引用本文复制引用
祝文轩,师全英,王向红,杨瑞利,程鑫颖,米思..辣椒素与槲皮苷通过EGFR/PI3K/Akt信号通路调节HepG2细胞脂质代谢作用机制[J].食品科学,2024,45(10):45-53,9.基金项目
河北省自然科学基金生物农业联合基金培育项目(C2022204222) （C2022204222）
国家自然科学基金青年科学基金项目(32202013) （32202013）
大学生创新创业训练计划项目(s202310086016) （s202310086016）
