食品科学2024，Vol.45Issue(10)：257-264,8.DOI:10.7506/spkx1002-6630-20231015-119
免疫亲和层析技术协同酶联免疫吸附法检测赭曲霉毒素A
Detection of Ochratoxin A Using Immunoaffinity Chromatography Combined with Enzyme-Linked Immunosorbent Assay
摘要
Abstract
Ochratoxin A(OTA)in samples was captured and concentrated by immunoaffinity chromatography(IAC)and detected by enzyme-linked immunosorbent assay(ELISA).The anti-OTA monoclonal antibodies used in IAC and ELISA came from different clone strains,belonging to different idiotypes,and there were differences between their selectivity for binding to OTA targets.The combined use of IAC and ELISA could effectively filter out the interference from OTA analogues,thus improving the specificity and sensitivity of immunological analysis.The limit of detection(LOD)for OTA in spiked samples was 0.2 ng/g,the quantification limit was 0.4 ng/g,and the average recovery was 75.9％.Although the recovery of the IAC-ELISA method was slightly lower than that of ELISA,the sensitivity was increased by 60 times.For 49 real samples,the proportion of samples that tested positive by this combined method was 100％consistent with the result of the national standard method,with a missed detection rate of 0％.Therefore,the developed method exhibited significant advantages in accuracy.As a comprehensive immunoassay,this method does not require any large-scale instruments or equipment,and has no strict requirement on the operating environment,thereby making it easy for grassroot laboratories to analyze OTA in samples.关键词
免疫亲和层析技术/酶联免疫吸附法/检测/赭曲霉毒素AKey words
immunoaffinity chromatography/enzyme-linked immunosorbent assay/detection/ochratoxin A分类
轻工纺织引用本文复制引用
张蕴哲,张先舟,袁耀武,张伟..免疫亲和层析技术协同酶联免疫吸附法检测赭曲霉毒素A[J].食品科学,2024,45(10):257-264,8.基金项目
国家自然科学基金面上项目(32172288 （）
31371772) （）
河北省自然科学基金重点项目(C2019204342) （C2019204342）
中央引导地方科技发展资金项目(236Z5502G) （236Z5502G）
