食品科学2024，Vol.45Issue(11)：24-30,7.DOI:10.7506/spkx1002-6630-20230904-023
基于转录组学探究高糖饮食诱导秀丽线虫衰老的分子机制
Transcriptomic Analysis of the Molecular Mechanism for High-Glucose Diet-Induced Aging of Caenorhabditis elegans
摘要
Abstract
The aim of this study was to uncover the molecular mechanism for high-sugar diet-induced aging of Caenorhabditis elegans.Changes in the gene expression profile of C.elegans induced by a high-glucose(HG)diet were explored by transcriptomics.The results showed that the expression of 4 183 genes in C.elegans significantly changed after HG diet consumption.In addition,Gene Ontology(GO)enrichment analysis showed that differentially expressed genes(DEGs)induced by HG diet treatment were significantly enriched in aging,unfolded protein binding,response to stress,autophagy,transferase activity,catalytic activity,and heat shock protein binding.Moreover,Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the DEGs were also significantly enriched in longevity regulation of C.elegans,autophagy regulation,pyruvate metabolism,the forkhead box O(FOXO)signaling pathway,glycolysis/gluconeogenesis,glutathione metabolism,and the transforming growth factor-β signaling pathway.Furthermore,we found that high-glucose diet up-regulated the expression of DEGs involved in the insulin/IGF-1 signaling(IIS)pathway and of DEGs associated with glycolipid metabolism,and down-regulated the expression of DEGs related to heat stress resistance and antioxidant regulation.This study provides a theoretical basis for further elucidation of the molecular mechanism underlying the effects of HG diet on the longevity and metabolism of C.elegans.关键词
高糖饮食/秀丽隐杆线虫/转录组学/衰老Key words
high-glucose diet/Caenorhabditis elegans/transcriptomics/aging分类
轻工业引用本文复制引用
李蓉,徐艳,缪园欣,陶明芳..基于转录组学探究高糖饮食诱导秀丽线虫衰老的分子机制[J].食品科学,2024,45(11):24-30,7.基金项目
湖北省科技厅重点研发计划项目(2022BCE057) （2022BCE057）
荆楚理工学院特色花卉生物育种湖北省工程中心项目(2023ZD006) （2023ZD006）
荆楚理工学院博士启动金项目(YY202403) （YY202403）
湖北省农业科学院青年科学基金项目(2024NKYJJ30) （2024NKYJJ30）
