食品科学2024，Vol.45Issue(11)：31-40,10.DOI:10.7506/spkx1002-6630-20230904-018
枸杞叶黄酮对HepG2细胞脂质代谢的影响及作用机制
Effects and Underlying Mechanisms of Lycium barbarum Leaves Flavonoids on Lipid Metabolism in HepG2 Cells
摘要
Abstract
The aim of this study was to investigate the impact of Lycium barbarum leaves flavonoids(LBLF)on lipid accumulation in normal and oleic acid-induced HepG2 cells.The experiment was divided into six groups as follows:blank,model,positive control,low-,medium-,and high-dose LBLF.Oil red O staining was performed to assess the aggregation of lipid droplets in each group,and lipid levels and oxidative stress indicators were measured.Finally,real-time polymerase chain reaction(real-time PCR)and Western blot were used to detect the expression of mRNAs and proteins associated with lipid synthesis.The results indicated that LBLF could decrease the increase in the levels of triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),malondialdehyde(MDA)and reactive oxygen species(ROS)induced by oleic acid in HepG2 cells.It also could increase high-density lipoprotein cholesterol(HDL-C),superoxide dismutase(SOD),glutathione,and catalase levels.Additionally,Western blot results suggested that LBLF intervention could up-regulate phosphorylated adenosine 5'-monophosphate(AMP)-activated protein kinase(p-AMPK)and AMPK levels,and down-regulate the protein expression of sterol regulatory element-binding protein 1c(SREBP-1c),peroxisome proliferator-activated receptor y(PPARy),and CCAAT enhancer binding protein alpha(CEBPα).Real-time PCR results indicated that LBLF intervention down-regulated the expression of genes such as ACC,FAS,SREBP-1c,SCD-1,PPARγ,and CEBPα.In conclusion,LBLF could partially alleviate lipid metabolism disorder in oleic acid-induced HepG2 cells by regulating the expression levels of lipid synthesis and oxidation-related genes.关键词
枸杞叶黄酮/HepG2细胞/脂质代谢/作用机制Key words
Lycium barbarum leaves flavonoids/HepG2 cells/lipid metabolism/action mechanism分类
轻工业引用本文复制引用
杨超,裴宇芳,孙霞芝,刘霜,张惠玲,范艳丽..枸杞叶黄酮对HepG2细胞脂质代谢的影响及作用机制[J].食品科学,2024,45(11):31-40,10.基金项目
宁夏回族自治区重点研发计划项目(2022BBF02006) （2022BBF02006）
