食品科学2024，Vol.45Issue(11)：41-51,11.DOI:10.7506/spkx1002-6630-20230905-030
羊血红蛋白水解物中新型抗氧化肽的纯化、鉴定和对H2O2损伤Caco-2细胞保护作用
Purification and Identification of Novel Antioxidant Peptides from Sheep Hemoglobin Hydrolysate and Their Protective Effect on H2O2-Induced Oxidative Injury in Caco-2 Cells
摘要
Abstract
In this study,antioxidant peptides derived from sheep hemoglobin were prepared from fresh sheep blood.Sephadex G-25 chromatography,DEAE Sephadex A-50 chromatography,reverse phase high performance liquid chromatography(RP-HPLC)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)were used to separate,purify,and identify peptides from the hydrolysate of sheep hemoglobin.The antioxidant activity of the obtained peptides was evaluated by their free radical scavenging capacity,stability against simulated gastrointestinal digestion and protective effect on H2O2-induced oxidative injury in Caco-2 cells.The results indicated that three novel antioxidant peptides,Ala-Tyr-Glu-Val-Asp(AYEVD),Phe-His-Thr-Met-Glu(FHTME)and Ser-Phe-Met-Tyr-Glu-Lys(SFMYEK),were purified and identified from the hydrolysate,with molecular masses of 595.60,663.74 and 803.92 Da,respectively.Among them,AYEVD had the strongest scavenging capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,and AYEVD showed stronger antioxidant activity after simulated gastrointestinal digestion.In addition,AYEVD inhibited H2O2-induced oxidative injury in Caco-2 cells,significantly reduced the accumulation of reactive oxygen species(ROS),suppressed early cell apoptosis and the formation of malondialdehyde(MDA),and increased the activity of intracellular antioxidant enzymes.Therefore,this study could provide a theoretical basis for the application of novel antioxidant peptides from sheep hemoglobin in functional foods.关键词
羊血红蛋白/抗氧化肽/纯化/鉴定/Caco-2细胞/细胞保护Key words
sheep hemoglobin/antioxidant peptide/purification/identification/Caco-2 cells/cytoprotection分类
轻工业引用本文复制引用
马泽浩,耿玉坤,王静云,卢士玲,曹逗逗,刘星语..羊血红蛋白水解物中新型抗氧化肽的纯化、鉴定和对H2O2损伤Caco-2细胞保护作用[J].食品科学,2024,45(11):41-51,11.基金项目
石河子大学青年创新培育人才项目(CXPY202108) （CXPY202108）
兵团指导性科技计划项目(2022ZD064) （2022ZD064）
五师科技计划项目(2023XM02) （2023XM02）
石河子大学高层次人才科研启动项目(RCZK202021) （RCZK202021）
