生物技术通报2024，Vol.40Issue(5)：74-83,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1148
基于SSR标记的文冠果遗传多样性分析及指纹图谱构建
Genetic Diversity Analysis and DNA Fingerprint Construction Based on SSR Markers for Xanthoceras sorbifolia
摘要
Abstract
[Objective]This work aims to quickly identify and utilize Xanthoceras sorbifolia germplasm resources,and explore the relationship of germplasm.[Method]Eleven primers with clear bands and good polymorphism were selected from 20 pairs of SSR primers.Fluorescence capillary electrophoresis technology was used for 29 X.sorbifolia varieties(lines)polymorphism detection.The neighbor-joining method was utilized for cluster analysis,while numerical and letter codes were assigned to create molecular identity cards.[Result]A total of 66 alleles were detected,and each pair of primers detected 3-10 alleles with an average of 6.The Shannon information index(I)varied from 1.723 to 0.349,with an average of 1.16.The polymorphic information content(PIC)revealed by different primers ranged from 0.276 to 0.841,with an average of 0.66.The observed heterozygosity(Ho)ranged from 0.137 to 0.958,with an average of 0.739.The expected heterozygosity(He)ranged from 0.187 to 0.79,with an average of 0.648.Among the 11 loci,7 loci had an average observed heterozygosity greater than the average expected heterozygosity.The genetic diversity coefficient varied from 0 to 1 with an average of 0.31.There were 15 individuals with a genetic similarity coefficient above 0.6,accounting for only 5.17%of the total data.At the genetic distance of 0.42,the 29 X.sorbifolia materials were divided into three categories by cluster analysis.Based on these results,the DNA fingerprint database composed of the digits 0 and 1 and the molecular identity card were established,which allowed unequivocal identification of all the varieties except a few unique ones.[Conclusion]This indicates that the genetic diversity of the 29 germplasm resources of X.sorbifolia is relatively high,but there is also a certain degree of inbreeding.Molecular identity cards for X.sorbifolia using SSR markers is simple and feasible,which may provide technical support and scientific basis for genetic identification,rights protection,identity validation,traceability management,and new variety breeding of X.sorbifolia varieties.关键词
文冠果/品种(系)/SSR荧光标记/遗传多样性/指纹图谱/分子身份证/聚类分析Key words
Xanthoceras sorbifolia/varieties(lines)/fluorescent labeled SSR markers/genetic diversity/fingerprints/molecular identity card/clustering analysis引用本文复制引用
李思琪,张文臣,杨柳,付庆新,洪新,张海旺..基于SSR标记的文冠果遗传多样性分析及指纹图谱构建[J].生物技术通报,2024,40(5):74-83,10.基金项目
中央财政林业科技推广示范项目(辽[2022]TG19号),辽宁省农业科学院旱地特色经济林学科建设计划项目(2019DD206530),辽宁省林业和草原局依托国家林草局科技创新平台研发项目(LLC[2023]21) （辽[2022]TG19号）
