子宫内膜异位症在位内膜上皮类器官模型的建立及鉴定OACSTPCD

Objective:To explore and verify the feasibility and influencing factors of constructing in-situ endometrial epithelial organoid model in human endometriosis patients.Methods:10 cases of fresh in-situ endometrial tissue from patients with ovarian endometriosis cyst diagnosed by laparoscopy and pathological diagnosis were selected.The in-situ endometrial tissue was digested into single cells by tissue digestion method.The human endometriosis eutop-ic epithelial organoids were cultured in Matrigel for 7 days.The model was identified by mor-phology,immunohistochemistry,HE staining and transmission electron microscopy.The diameter changes of in-place endometrial epithelial organoids were observed after the intervention of es-trogen at 1nmol/L,5nmol/L and 10nmol/L,and the role of estrogen in the construction of in-place endometrial epithelial organoids was initially discussed.Results:EMs endometrial or-ganoids were successfully constructed in the endometrial tissues of 10 patients.Optical micros-copy showed that there were a considerable number of endometrial organoids in the endometrial tissues,and their morphology was similar to circular 3D structure with glandular structures a-round them.Immunohistochemistry showed that epidermal markers and estrogen receptor expres-sion were positive in endometrium-derived epithelium.HE staining showed that the on-site en-dometrial epithelial organoids constructed under high power microscope were arranged in a glan-dular structure,but the glands were arranged sparsely and irregularly,and some cells in the cav-ity formed comparttures similar to the glandular epithelium structure of the original tissue.Transmission electron microscopy(TEM)showed that the internal ultrastructure of in situ endo-metrial epithelial organoids was highly similar to that of the original in situ endometrial tissues.When different concentrations of estradiol were added to the medium,the organoid diameter was significantly larger than that of the control group on day 6 after the intervention of 10nmol/L es-trogen concentration(P<0.05).Conclusion:The in-situ endometrial epithelial organoid model of EMs successfully constructed in vitro has epithelial characteristics,and the structure and pathological characteristics of the source tissue can be highly reproduced when compared with the original in-situ endometrial tissue,Modeling of in-place endometrial tissue as an alternative to human endometriosis for EMs disease drug intervention and basic experimental research.